Closed IgnaciaMeza closed 5 years ago
Hey there, I have been trying to use your R package for qPCR analysis. But R shows an error that the “pcr_analyze” function was not found.
So far I've done:
# install package: install.packages('pcr') # load required libraries: library(pcr) # default mode delta_delta_ct ## locate and read raw ct data fl <- system.file('extdata', 'ct1.csv', package = 'pcr') ct1 <- read.csv(fl) ## add grouping variable group_var <- rep(c('brain', 'kidney'), each = 6) str(ct1) # calculate all values and errors in one step res <- pcr_analyze(ct1, group_var = group_var, reference_gene = 'GAPDH', reference_group = 'brain')
Here is when I get the error "Error in pcr_analyze(ct1, group_var = group_var, reference_gene = "GAPDH", : could not find function "pcr_analyze""
I am very new to R coding, so I might need a more thorough explanation than experienced users!
Thanks!..
Hi @IgnaciaMeza I edited the code in your comment so that it should run without errors. Please try it and report back if you got any errors.
Hey there, I have been trying to use your R package for qPCR analysis. But R shows an error that the “pcr_analyze” function was not found.
So far I've done:
Here is when I get the error "Error in pcr_analyze(ct1, group_var = group_var, reference_gene = "GAPDH", : could not find function "pcr_analyze""
I am very new to R coding, so I might need a more thorough explanation than experienced users!
Thanks!..