Paraudie commented 4 years ago

Hi, I have difficulty when I get to that level: "res<-pcr_analyze(ct1,group_var=group,reference_gene='RPS7',reference_group='Kisumu') res <- pcr_analyze(ct1,group_var = group_var,reference_gene='RPS7',reference_group='Kisumu')";

here's what I get as a result; > res<-pcr_analyze(ct1,group_var=group,reference_gene='RPS7',reference_group='Kisumu') Error in pcr_analyze(ct1, group_var = group, reference_gene = "RPS7", : could not find function "pcr_analyze"

Note: when I install the package and load "library 'pcr', this appears:

library(pcr) Erreur : package or namespace load failed for ‘pcr’: .onLoad a échoué dans loadNamespace() pour 'pillar', détails : appel : utils::packageVersion("vctrs") erreur : package ‘vctrs’ not found

Thanks for helping me

MahShaaban commented 4 years ago

Hi,

You probably need to install vctrs first. And try to load the pcr package, it should load without any error messages before you can use it.

theneti3 commented 3 years ago

Hi,

I have the same problem:

fl <- system.file('extdata', 'ct1.csv', package = 'pcr') ct1 <- read.csv(fl)

add grouping variable

group_var <- rep(c('brain', 'kidney'), each = 6) str(ct1)

calculate all values and errors in one step

res <- pcr_analyze(ct1, group_var = group_var, reference_gene = 'GAPDH', reference_group = 'brain')

--> Fehler in pcr_analyze(ct1, group_var = group_var, reference_gene = "GAPDH", : konnte Funktion "pcr_analyze" nicht finden

Can anyone help? thanks

MahShaaban commented 3 years ago

You need to make sure first that the package is installed properly and loaded before you can run this code.

MahShaaban / pcr

pcr #18

add grouping variable

calculate all values and errors in one step