Workflow abruptly stops

[nurudeen95]

I am trying AlphaDIA for the first time for 72 .raw files for a library free search. However, when I start the run, it stops within seconds with the following report below:

Method Setup CPU: 0.69% RAM: 11.48 GB / 63.63 GB (18.05%) Unknown arguments: ['A\20240416_ZOEIb_20240329_ProjectA_Serum\Samples\AlphaDIA_Report_061724\config.yaml'] usage: alphadia.exe [-h] [--version] [--output [OUTPUT]] [--file FILE] [--directory DIRECTORY] [--regex [REGEX]] [--library [LIBRARY]] [--fasta FASTA] [--config [CONFIG]] [--wsl] [--config-dict [CONFIG_DICT]]

Search DIA experiments with alphaDIA

options: -h, --help show this help message and exit --version, -v Print version and exit --output [OUTPUT], -o [OUTPUT] Output directory --file FILE, -f FILE Raw data input files. --directory DIRECTORY, -d DIRECTORY Directory containing raw data input files. --regex [REGEX], -r [REGEX] Regex to match raw files in directory. --library [LIBRARY], -l [LIBRARY] Spectral library. --fasta FASTA Fasta file(s) used to generate or annotate the spectral library. --config [CONFIG], -c [CONFIG] Config yaml which will be used to update the default config. --wsl, -w Set if running on Windows Subsystem for Linux. --config-dict [CONFIG_DICT] Python Dict which will be used to update the default config. WARNING:root:WARNING: Temp mmap arrays were written to C:\Users\DiscoveryProteomics\AppData\Local\Temp\7\temp_mmap_6elyg_up. Cleanup of this folder is OS dependant, and might need to be triggered manually! Current space: 1,847,586,598,912

What could be the issue here?

I also attached a picture of the settings used. Thank you

[jalew188]

@GeorgWa Potential bug on Windows: path with spaces

[GeorgWa]

Hi, yes, this seems to be an issue with parsing a path space in hte path. A quickfix would be to change the output folder to a location where there are no spaces in the path. We can fix this with the next version.