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MannLabs / alphadia

modular & open DIA search
https://alphadia.readthedocs.io
Apache License 2.0
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No objects to concatenate (astral) #367

Open wangss3 opened 5 days ago

wangss3 commented 5 days ago

Hi,

I downloaded astral raw files and analyzed wiht library free search. And here's the log file. log.txt

Best, Ko

GeorgWa commented 2 days ago

Hi,

it looks like there was an error with the rt limit for the optimization. The RT limits become to close after generating the MBR library and alphaDIA can't extract full XICs anymore.

We will fix this and include it in the next version.

As a quick fix, set the target_rt_tolerance = 50in the second search. This should prevent the error and give the same results.

Best, Georg