MariaNattestad / Assemblytics

Assemblytics is a bioinformatics tool to detect and analyze structural variants from a genome assembly by comparing it to a reference genome.
http://assemblytics.com
MIT License
135 stars 28 forks source link

No file uploaded #49

Closed weilu1998 closed 6 months ago

weilu1998 commented 1 year ago

Hi,

I think the server is filled again. I keep getting the error no file uploaded.

Thanks, Wei

mschatz commented 1 year ago

Cleaning up now!

On Tue, Oct 25, 2022 at 5:28 PM Phragmites @.***> wrote:

Hi,

I think the server is filled again. I keep getting the error no file uploaded.

Thanks, Wei

— Reply to this email directly, view it on GitHub https://github.com/MariaNattestad/Assemblytics/issues/49, or unsubscribe https://github.com/notifications/unsubscribe-auth/AABP346QDCRKGL7NYPFM4TLWFBGGXANCNFSM6AAAAAAROL2GNQ . You are receiving this because you are subscribed to this thread.Message ID: @.***>

Daeap commented 1 year ago

Hi, just wanted to say the same is happening again right now.

Thank you for your time,

David

matt-shenton commented 1 year ago

HI there,

many thanks for the assemblytics tool.

I'm also having file upload trouble.

Best wishes

Matt Shenton

robackem commented 1 year ago

Hello, just wanted to make a note that this is happening again.

Many thanks!

mschatz commented 1 year ago

Please try again. It should be fixed now!

Mike

On Wed, Mar 15, 2023 at 9:54 PM robackem @.***> wrote:

Hello, just wanted to make a note that this is happening again.

Many thanks!

— Reply to this email directly, view it on GitHub https://github.com/MariaNattestad/Assemblytics/issues/49#issuecomment-1471158236, or unsubscribe https://github.com/notifications/unsubscribe-auth/AABP34Y2G4NC4UTXXMHTNCDW4JXFXANCNFSM6AAAAAAROL2GNQ . You are receiving this because you commented.Message ID: @.***>

ethan-baldwin commented 1 year ago

Hi, it seems like the server is filled up again. Thanks!

Gon1976 commented 6 months ago

Hi Mike, could be web server full? I have the error "no file upload" Thanks

MariaNattestad commented 6 months ago

I just checked. The server is not full, and I was able to upload a file myself. Please try again.

On Wed, Mar 13, 2024 at 6:09 AM Gon1976 @.***> wrote:

Hi Mike, could be web server full? I have the error "no file upload" Thanks

— Reply to this email directly, view it on GitHub https://github.com/MariaNattestad/Assemblytics/issues/49#issuecomment-1994371138, or unsubscribe https://github.com/notifications/unsubscribe-auth/AB4W4PJVHDJ7EI6FQDPIXFLYYBFZHAVCNFSM6AAAAAAROL2GNSVHI2DSMVQWIX3LMV43OSLTON2WKQ3PNVWWK3TUHMYTSOJUGM3TCMJTHA . You are receiving this because you modified the open/close state.Message ID: @.***>

Gon1976 commented 6 months ago

I change my .delta file and start working and this error appear:

Starting unique anchor filtering.

Step 1: Assemblytics_uniq_anchor.py failed: Possible problem with Python or Python packages on server.

The .delta file with "no upload" message it's a self-self comparison. My nucmer command was: nucmer --maxmatch -l 100 -c 500 H1.fasta H1.fasta -p H1vsH1

MariaNattestad commented 6 months ago

Just edit the first lines of the .delta file to give them different file names. The two filenames are assumed to be different in the code.

On Wed, Mar 13, 2024 at 12:28 PM Gon1976 @.***> wrote:

I change my .delta file and start working and this error appear:

Starting unique anchor filtering.

Step 1: Assemblytics_uniq_anchor.py failed: Possible problem with Python or Python packages on server.

The .delta file with "no upload" message it's a self-self comparison. My nucmer command was: nucmer --maxmatch -l 100 -c 500 H1.fasta H1.fasta -p H1vsH1

— Reply to this email directly, view it on GitHub https://github.com/MariaNattestad/Assemblytics/issues/49#issuecomment-1995494719, or unsubscribe https://github.com/notifications/unsubscribe-auth/AB4W4PKS5Q4YKIIF7BRVBRDYYCSHDAVCNFSM6AAAAAAROL2GNSVHI2DSMVQWIX3LMV43OSLTON2WKQ3PNVWWK3TUHMYTSOJVGQ4TINZRHE . You are receiving this because you modified the open/close state.Message ID: @.***>

Gon1976 commented 6 months ago

Ok, the upload error continue changing the filenames. In the second case, i can upload the delta file (it different comparison) and I have another error: Starting unique anchor filtering.

Step 1: Assemblytics_uniq_anchor.py failed: Possible problem with Python or Python packages on server.

I add my two delta files deltafiles.zip

MariaNattestad commented 6 months ago

Okay I looked at your delta files, and they have weird formatting.

Screenshot 2024-03-14 at 10 22 31 AM Screenshot 2024-03-14 at 10 22 24 AM

See line 3-4 and again in other places in the file:

Screenshot 2024-03-14 at 10 23 33 AM

See a sample delta file here: http://assemblytics.com/sample.delta

I'm guessing you tried to change the file programmatically and accidentally added some newlines where they shouldn't be. When you fix this, please take a close look at your file in a text editor and also try running it through the MUMmer tool suite first before reporting problems in Assemblytics, since it will be faster for you to check that these files are valid using e.g. MUMmer's "show-aligns" or "coords" commands. Once those are working and it looks okay , then you can try Assemblytics again. Good luck!

MariaNattestad commented 6 months ago

Sorry this issue is totally different, we should have opened a new issue to deal with your problems @Gon1976. Please opena new issue now instead of replying here and just link to the comments above if you need to.

brcliver commented 4 months ago

Hi there, any chance the server is full?

MariaNattestad commented 4 months ago

Thanks, yes it was. Fixed! Also I made a cron job that should hopefully prevent this from happening again...we'll see if it works this time.

On Wed, May 15, 2024 at 8:38 AM brcliver @.***> wrote:

Hi there, any chance the server is full?

— Reply to this email directly, view it on GitHub https://github.com/MariaNattestad/Assemblytics/issues/49#issuecomment-2112882098, or unsubscribe https://github.com/notifications/unsubscribe-auth/AB4W4PPVQYHRTTOV4HPQLMDZCN6PPAVCNFSM6AAAAAAROL2GNSVHI2DSMVQWIX3LMV43OSLTON2WKQ3PNVWWK3TUHMZDCMJSHA4DEMBZHA . You are receiving this because you modified the open/close state.Message ID: @.***>