When using this code for the first time, usually you might encounter the following three main problematics:
1. Compilation of FLIM Analysis Functions and Input Parameters
To successfully utilize this code, you'll need to ensure that you compile all the FLIM analysis functions and input parameters.
2. File Naming Convention
For correct data interpretation and analysis, it's crucial to adhere to the following naming convention for your data files:
Date of the experiment in the format YYYYMMDD.
Sample 1: Name or identifier for the sample (no underscores allowed).
1: Number indicating the replica of the experiment.
The naming format helps the code associate different samples and replicas within the same experimental context.
Example: 20230830_Sample1_1.R64
3. No Phasor detected
If you receive a signal but not a phasor, you can adjust the following input parameters:
ROI_min_extent (default set to 5000): Determines the minimum extent in pixels/datapoints in the phasor plot to consider a Region of Interest (ROI) worth analyzing.
phasor_min_extent (default set to 1000): Determines the minimum extent in pixels/datapoints in the phasor plot to consider a phasor worth analyzing.
Proper configuration of initial parameters, including file format, is essential for obtaining meaningful results from your data.
Please ensure that you follow these guidelines to make the most of the code and resolve issues that may arise during your initial usage. If anything else comes up don't hesitate to get in touch.
When using this code for the first time, usually you might encounter the following three main problematics:
1. Compilation of FLIM Analysis Functions and Input Parameters
To successfully utilize this code, you'll need to ensure that you compile all the FLIM analysis functions and input parameters.
2. File Naming Convention
For correct data interpretation and analysis, it's crucial to adhere to the following naming convention for your data files:
The naming format helps the code associate different samples and replicas within the same experimental context.
Example:
20230830_Sample1_1.R643. No Phasor detected
If you receive a signal but not a phasor, you can adjust the following input parameters:
Please ensure that you follow these guidelines to make the most of the code and resolve issues that may arise during your initial usage. If anything else comes up don't hesitate to get in touch.