while running PrimedRPA (downloaded from Bioconda) we are encountering following error
$ PrimedRPA --InputFile HBV.fasta --RunID HBV_1
-------------------------------------------
----------------PrimedRPA------------------
-----Finding RPA Primer and Probe Sets-----
-------------Higgins M et al.--------------
-------------------------------------------
Generating Alignment Summary
multiprocessing.pool.RemoteTraceback:
"""
Traceback (most recent call last):
File "/home/obront/miniforge3/envs/primedrpa/lib/python3.8/multiprocessing/pool.py", line 125, in worker
result = (True, func(*args, **kwds))
File "/home/obront/miniforge3/envs/primedrpa/lib/python3.8/multiprocessing/pool.py", line 51, in starmapstar
return list(itertools.starmap(args[0], args[1]))
File "/home/obront/miniforge3/envs/primedrpa/bin/PrimedRPA", line 438, in CreatingInputHomologyDF
AlignedDF = AlignedDF.append({'Index_Pos':seqindex,'Nucleotide':MostCommonN,'IdentityScore':NRepresentation},ignore_index=True)
File "/home/obront/miniforge3/envs/primedrpa/lib/python3.8/site-packages/pandas/core/generic.py", line 5989, in __getattr__
return object.__getattribute__(self, name)
AttributeError: 'DataFrame' object has no attribute 'append'
"""
The above exception was the direct cause of the following exception:
Traceback (most recent call last):
File "/home/obront/miniforge3/envs/primedrpa/bin/PrimedRPA", line 1046, in <module>
CheckingAlignedOutputFile(AllParameter)
File "/home/obront/miniforge3/envs/primedrpa/bin/PrimedRPA", line 806, in CheckingAlignedOutputFile
AlignedDFMultiThreadOupt = pool.starmap(CreatingInputHomologyDF,MTDFOVI)
File "/home/obront/miniforge3/envs/primedrpa/lib/python3.8/multiprocessing/pool.py", line 372, in starmap
return self._map_async(func, iterable, starmapstar, chunksize).get()
File "/home/obront/miniforge3/envs/primedrpa/lib/python3.8/multiprocessing/pool.py", line 771, in get
raise self._value
AttributeError: 'DataFrame' object has no attribute 'append'
looks like this is related to issue mentioned here
while running PrimedRPA (downloaded from Bioconda) we are encountering following error
looks like this is related to issue mentioned here
https://stackoverflow.com/questions/75956209/error-dataframe-object-has-no-attribute-append