Index Error: list index out of range

[JamesGerardMann]

I've come across this error when trying to run the program as well.

Traceback (most recent call last): File "/home/jmann/anaconda3/lib/python3.7/multiprocessing/pool.py", line 121, in worker result = (True, func(*args, **kwds)) File "/home/jmann/anaconda3/lib/python3.7/multiprocessing/pool.py", line 47, in starmapstar return list(itertools.starmap(args[0], args[1])) File "/home/jmann/anaconda3/bin/PrimedRPA", line 541, in IndentifyingAndFilteringOligos MaxBackgroundScoreBindingScore, MaxScoreBackSeq, HardFailBool = BlastnBackgroundCheck(NucleotideSeq, AllParameter) File "/home/jmann/anaconda3/bin/PrimedRPA", line 203, in BlastnBackgroundCheck BackgroundMaxBindingPercentage, BackgroundMaxBindingString, BackgroundHardFail, BackgroundString = SSIdentification(seq, ComplementSeq, False, ShorterBackground) File "/home/jmann/anaconda3/bin/PrimedRPA", line 324, in SSIdentification if NucDict[DyanimicSeqList[StringPos]] == FixedSeqList[StringPos]: IndexError: list index out of range """

The above exception was the direct cause of the following exception:

Traceback (most recent call last): File "/home/jmann/anaconda3/bin/PrimedRPA", line 1034, in CheckingAlignedOutputFile(AllParameter) File "/home/jmann/anaconda3/bin/PrimedRPA", line 810, in CheckingAlignedOutputFile PotentialPrimerProbeOut = pool.starmap(IndentifyingAndFilteringOligos,PrimerProbeCheckParallelInput) File "/home/jmann/anaconda3/lib/python3.7/multiprocessing/pool.py", line 276, in starmap return self._map_async(func, iterable, starmapstar, chunksize).get() File "/home/jmann/anaconda3/lib/python3.7/multiprocessing/pool.py", line 657, in get raise self._value IndexError: list index out of range

[JamesGerardMann]

This only seems to happen when my blast_db.fasta exceeds 3500 sequences.

[MatthewHiggins2017]

Dear James,

I apologies for my slow response and this bug should be fixed in PrimedRPA v1.0.2 now available through bioconda.

[JamesGerardMann]

Thanks!