MatthewHiggins2017 / bioconda-PrimedRPA

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PrimedRPA_Walkthrough_Challenge #6

Closed noutin2020 closed 3 years ago

noutin2020 commented 3 years ago

Hi Dear seniors.

I am using PrimedRPA to create and filter RPA primers and probes on my Ubuntu 16.04 LTS I therefore followed PrimedRPA Walkthrough available on https://github.com/MatthewHiggins2017/bioconda-PrimedRPA/wiki/WalkThrough. I previously installed miniconda3 on my Linux (Ubuntu 16.04) and ran the different command lines recommended in that Step-by-step procedure. Furthermore, I checked out the different examples given by the author. Unfortunately, I encountered the following challenge :

mifer@fmichodigni:~/Desktop/Nasser_RPA$ PrimedRPA PrimedRPA_Parameters.txt PrimedRPA: command not found mifer@fmichodigni:~/Desktop/Nasser_RPA$

This my home Directory: mifer@fmichodigni:~/Desktop/Nasser_RPA$ ls EF179169.1?report=fasta PrimedRPA-master GCF_000896435.1_ViralProj76727_genomic.fna PrimedRPA_Parameters.txt

Kindly assist me to find out the right command line or advise on this matter. I look forward to learning from. Cheers, Noutin Fernand, PhD fellow in Molecular Biology & Biotechnology.

noutin2020 commented 3 years ago

The issue was solved after revising the installation of miniconda3 package with Dr. Caleb kibet @ICIPE, Kenya.

I am grateful to him and to Higgins M et al. for coming up with such an illuminative resource.