UnboundLocalError

[thawatchai-chi]

Hi, while I was following the step-by-step walkthrough, I encountered the following error:

Generating Alignment Summary Traceback (most recent call last): File "", line 1, in File "/Users/Benz/opt/miniconda3/lib/python3.8/multiprocessing/spawn.py", line 116, in spawn_main exitcode = _main(fd, parent_sentinel) File "/Users/Benz/opt/miniconda3/lib/python3.8/multiprocessing/spawn.py", line 125, in _main prepare(preparation_data) File "/Users/Benz/opt/miniconda3/lib/python3.8/multiprocessing/spawn.py", line 236, in prepare _fixup_main_from_path(data['init_main_from_path']) File "/Users/Benz/opt/miniconda3/lib/python3.8/multiprocessing/spawn.py", line 287, in _fixup_main_from_path main_content = runpy.run_path(main_path, File "/Users/Benz/opt/miniconda3/lib/python3.8/runpy.py", line 265, in run_path return _run_module_code(code, init_globals, run_name, File "/Users/Benz/opt/miniconda3/lib/python3.8/runpy.py", line 97, in _run_module_code _run_code(code, mod_globals, init_globals, File "/Users/Benz/opt/miniconda3/lib/python3.8/runpy.py", line 87, in _run_code exec(code, run_globals) File "/Users/Benz/opt/miniconda3/bin/PrimedRPA", line 1046, in CheckingAlignedOutputFile(AllParameter) File "/Users/Benz/opt/miniconda3/bin/PrimedRPA", line 807, in CheckingAlignedOutputFile MergedAlignedDF = pd.concat(AlignedDFMultiThreadOupt).reset_index().drop(['index'],axis=1) UnboundLocalError: local variable 'AlignedDFMultiThreadOupt' referenced before assignment

 I already made sure that I installed the latest version of all dependencies (including samtools) and have tried full anaconda, mini anaconda, and using virtual environment. Does anyone have an idea where I might have gone wrong? Thank you in advance.

[eandrusz]

I have the same error!

Generating Alignment Summary Traceback (most recent call last): File "", line 1, in File "/Users/elizabethandruszkiewicz/opt/anaconda2/envs/primed_env/lib/python3.9/multiprocessing/spawn.py", line 116, in spawn_main exitcode = _main(fd, parent_sentinel) File "/Users/elizabethandruszkiewicz/opt/anaconda2/envs/primed_env/lib/python3.9/multiprocessing/spawn.py", line 125, in _main prepare(preparation_data) File "/Users/elizabethandruszkiewicz/opt/anaconda2/envs/primed_env/lib/python3.9/multiprocessing/spawn.py", line 236, in prepare _fixup_main_from_path(data['init_main_from_path']) File "/Users/elizabethandruszkiewicz/opt/anaconda2/envs/primed_env/lib/python3.9/multiprocessing/spawn.py", line 287, in _fixup_main_from_path main_content = runpy.run_path(main_path, File "/Users/elizabethandruszkiewicz/opt/anaconda2/envs/primed_env/lib/python3.9/runpy.py", line 268, in run_path return _run_module_code(code, init_globals, run_name, File "/Users/elizabethandruszkiewicz/opt/anaconda2/envs/primed_env/lib/python3.9/runpy.py", line 97, in _run_module_code _run_code(code, mod_globals, init_globals, File "/Users/elizabethandruszkiewicz/opt/anaconda2/envs/primed_env/lib/python3.9/runpy.py", line 87, in _run_code exec(code, run_globals) File "/Users/elizabethandruszkiewicz/opt/anaconda2/envs/primed_env/bin/PrimedRPA", line 1046, in CheckingAlignedOutputFile(AllParameter) File "/Users/elizabethandruszkiewicz/opt/anaconda2/envs/primed_env/bin/PrimedRPA", line 807, in CheckingAlignedOutputFile MergedAlignedDF = pd.concat(AlignedDFMultiThreadOupt).reset_index().drop(['index'],axis=1) UnboundLocalError: local variable 'AlignedDFMultiThreadOupt' referenced before assignment

[na-89]

I also encountered the same problem, have you solved your problem?

[thawatchai-chi]

Hey, I solved the problem by downgrading the Python. I forgot which version I downgraded it to (haven’t used it for a while), but I’ll take a look and let you know.

Cheers, Benz

Sent from my iPhone

On 12 Feb 2022, at 09:55, na-89 @.***> wrote:

 I also encountered the same problem, have you solved your problem?

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[na-89]

Hi，Benz: Thanks for the reply, I use the 3.6 version of python, it seems to be able to run successfully ！

Best regards! Nana