Exception in thread "main" java.lang.NullPointerException
at umac.guava.diff.DifferentialOutputFiles.writeSummary(DifferentialOutputFiles.java:143)
at umac.guava.diff.DifferentialAnalysisWorkflow.startCommandlineDifferentialAnalysis(DifferentialAnalysisWorkflow.java:148)
at umac.guava.commandline.CommandlineWorkflow.prepareGdiffCommandlineWorkflow(CommandlineWorkflow.java:101)
at umac.guava.commandline.CommandlineWorkflow.startCommandlineFlow(CommandlineWorkflow.java:38)
at umac.guava.GUAVA.runGUAVAcommandline(GUAVA.java:83)
at umac.guava.GUAVA.main(GUAVA.java:64)
When trying to run guava from the command line, we get:
$ guava.sh gdiff -n deejtest -o . -files testing.txt -g hg19
Exception in thread "main" java.lang.NullPointerException at umac.guava.diff.DifferentialOutputFiles.writeSummary(DifferentialOutputFiles.java:143) at umac.guava.diff.DifferentialAnalysisWorkflow.startCommandlineDifferentialAnalysis(DifferentialAnalysisWorkflow.java:148) at umac.guava.commandline.CommandlineWorkflow.prepareGdiffCommandlineWorkflow(CommandlineWorkflow.java:101) at umac.guava.commandline.CommandlineWorkflow.startCommandlineFlow(CommandlineWorkflow.java:38) at umac.guava.GUAVA.runGUAVAcommandline(GUAVA.java:83) at umac.guava.GUAVA.main(GUAVA.java:64)
$ cat testing.txt DMSO_Rep1_R1_aligned_ATACseq.bam
$ ls -la DMSO_Rep1_R1_aligned_ATACseq.bam -rw-rw-r-- 1 deej deej 274174803 Mar 4 15:33 DMSO_Rep1_R1_aligned_ATACseq.bam
$ java -version java version "1.8.0_191" Java(TM) SE Runtime Environment (build 1.8.0_191-b12) Java HotSpot(TM) 64-Bit Server VM (build 25.191-b12, mixed mode)
$ cat deejtest_GUAVA_Differental_analysis/deejtest_log.txt #######################################################
Project summary
Name of the project: deejtest
Genome build: hg19
Pvalue: 0.05
Foldchange: 2.0
Upstream: 5000
Downstream: 3000
Input files
Any thoughts on where to start looking?
Thank you