AMR++ is a bioinformatic pipeline meant to aid in the analysis of raw sequencing reads to characterize the profile of antimicrobial resistance genes, or resistome.
I have been encountering an issue recently running AMR++ SNP verification with metagenomic data. A sample stopped execution at the runsnp process with the following message:
Command error:
concurrent.futures.process._RemoteTraceback:
"""
Traceback (most recent call last):
File "/usr/local/lib/python3.8/concurrent/futures/process.py", line 239, in _process_worker
r = call_item.fn(*call_item.args, **call_item.kwargs)
File "/usr/local/lib/python3.8/concurrent/futures/process.py", line 198, in _process_chunk
return [fn(*args) for args in chunk]
File "/usr/local/lib/python3.8/concurrent/futures/process.py", line 198, in <listcomp>
return [fn(*args) for args in chunk]
File "/opt/analysis/nextflow-bin/SNP_Verification.py", line 229, in iterate
verify(read, gene_variant, config)
File "/opt/analysis/nextflow-bin/SNP_Verification_Processes/__init__.py", line 49, in verify
nTupleCheck(read, gene, mapOfInterest, seqOfInterest, config)
File "/opt/analysis/nextflow-bin/SNP_Verification_Processes/nTupleCheck.py", line 171, in nTupleCheck
if mt == seqOfInterest[queryIndex]:
TypeError: string indices must be integers
"""
The above exception was the direct cause of the following exception:
Traceback (most recent call last):
File "/opt/analysis/nextflow-bin/SNP_Verification.py", line 353, in <module>
main()
File "/opt/analysis/nextflow-bin/SNP_Verification.py", line 348, in main
[gene_variant_dict.update(r) for r in results]
File "/opt/analysis/nextflow-bin/SNP_Verification.py", line 348, in <listcomp>
[gene_variant_dict.update(r) for r in results]
File "/usr/local/lib/python3.8/concurrent/futures/process.py", line 484, in _chain_from_iterable_of_lists
for element in iterable:
File "/usr/local/lib/python3.8/concurrent/futures/_base.py", line 619, in result_iterator
yield fs.pop().result()
File "/usr/local/lib/python3.8/concurrent/futures/_base.py", line 437, in result
return self.__get_result()
File "/usr/local/lib/python3.8/concurrent/futures/_base.py", line 389, in __get_result
raise self._exception
TypeError: string indices must be integers
Upon closer inspection of SNP_Verification.py, I have found that this particular error seems to occur when queryIndex at this line is set to None. I set some print statements for mapofInterest, seqOfInterest, and queryIndex in the loop this line is included in and took a screenshot of the output. I found that this loop is looped for a large number of times (over 265 times) for a specific sequence, before the value 11 for key 2251 in mapOfInterest below is set to None:
I am not quite sure why this behavior is happening, and would greatly appreciate any help with the matter.
Hello,
I have been encountering an issue recently running AMR++ SNP verification with metagenomic data. A sample stopped execution at the runsnp process with the following message:
Upon closer inspection of SNP_Verification.py, I have found that this particular error seems to occur when
queryIndex
at this line is set toNone
. I set some print statements formapofInterest
,seqOfInterest
, andqueryIndex
in the loop this line is included in and took a screenshot of the output. I found that this loop is looped for a large number of times (over 265 times) for a specific sequence, before the value 11 for key 2251 inmapOfInterest
below is set toNone
:I am not quite sure why this behavior is happening, and would greatly appreciate any help with the matter.
Thank you!