Hi, Mike,
I used ShortStack4 to annotate miRNA in animal, then I got 2541 sRNA, but only 188 passed all criteria defined as miRNA, others are more than 300nt.
I want to know why so many too long hairpin. Can i adjust the predicted hairpin length by some parameters? And how can i know the unreliable sRNA not satisfied which criteria from the output?
Hi, Mike, I used ShortStack4 to annotate miRNA in animal, then I got 2541 sRNA, but only 188 passed all criteria defined as miRNA, others are more than 300nt. I want to know why so many too long hairpin. Can i adjust the predicted hairpin length by some parameters? And how can i know the unreliable sRNA not satisfied which criteria from the output?
Thanks, Yu