MikeAxtell / ShortStack

ShortStack: Comprehensive annotation and quantification of small RNA genes
MIT License
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Which version of the ShortStack is selected to predict miRNAs in long hairpin structure #93

Closed Rease123 closed 3 years ago

Rease123 commented 5 years ago

Hello, The species I studied (animal) had many conserved miRNAs with long hairpin structure because it was the first identification. Please, which version of ShortStack should I use? I want to generate a file for the inverted repeats with reference to your 2013 publication, but I can't find the script ‘invert_it.pl’. Thanks,

MikeAxtell commented 3 years ago

Sorry to leave this here for over a year. I'm just catching up with open issues now. invert_it.pl is obsolete for a long time now .. the most recent version of ShortStack does not use that tool anymore. The 2013 paper is a fair bit of date I'm afraid. As with most software, your best bet is to use the most recent release of the tool. Hope this helps.