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Molmed / fastq_demux

Demultiplex Illumina FASTQ files
GNU General Public License v3.0
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Is it possible to allow mismatches? #11

Closed Yyeserin closed 1 year ago

Yyeserin commented 1 year ago

Hello,

I wonder if the software allows some mismatches between the barcodes in the list and on the headers? E.g. allowing 1 bp mismatch? I couldn't see such an option on the website. But maybe it is there.

Best, Yeserin.

b97pla commented 1 year ago

Hi!

Yes, it is now possible to allow mismatches in the index. Just use the --mismatches N parameter, where N is the number of mismatches to allow. Note that you will get an exception in case there are index collisions as a consequence.

See also fastq_demux --help