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Molmed / fastq_demux

Demultiplex Illumina FASTQ files
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All sequences to "barcode_Unknown" fastq--not demultiplexed #13

Open Rhizae42 opened 1 year ago

Rhizae42 commented 1 year ago

Sample sheet columns in the following order: sample ID, i7, i5

I've tried i5 as both the forward and reverse complement.

Sequences are paired end from NextSeq550. fastq.gz files include separate R1 and R2 files.

Why are they all going to barcode_Unknown fastq files?

alexell22 commented 7 months ago

Hey! have you managed to solve this issue? I have the same thing :(

Rhizae42 commented 7 months ago

Yes. The sequencer did not retain barcode information on the sequences. I had to get a program that could open any size text document to see it

Sarah Dean, PhD

Assay Development Scientist, Molecular Oncology

TriCore Reference Laboratories1001 Woodward Pl NEAlbuquerque NM 87102

Adjunct Faculty, Math Science and Engineering

Central New Mexico Community College

Max Salazar Hall 563

525 Buena Vista SE Albuquerque NM, 87106

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On Sat, Dec 30, 2023 at 12:25 PM alexell22 @.***> wrote:

Hey! have you managed to solve this issue? I have the same thing :(

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