MonashBioinformaticsPlatform / RNAsik-pipe

RNAsik - more than just a pipeline
https://monashbioinformaticsplatform.github.io/RNAsik-pipe/
Apache License 2.0
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Determine if qualimap results are misleading, if so remove/replace it #43

Open pansapiens opened 5 years ago

pansapiens commented 5 years ago

It's not clear how to interpret the qualimap (2.2.2b) report. The total number of mapped reads reported by qualimap seems to be <~ 10 % of the mapped reads, and the number of reads reported by qualimap as mapping to "Intergenic" features are < ~10% of the number of reads that featureCounts reports mapping to "Unassigned_NoFeatures", even though these would be expected to be similar values.

Maybe it's subsampling ?