tinyRNA provides an all-in-one solution for precision analysis of sRNA-seq data. At the core of tinyRNA is a highly flexible counting utility, tiny-count, that allows for hierarchical assignment of reads to features based on positional information, extent of feature overlap, 5’ nucleotide, length, and strandedness.
Overlap selector definitions will be extended to optionally accept 2 numerical parameters. These will allow users to create rules that shift the 5'/3' ends of matching features by the specified number of bases.
selector, M, N
M = shift value for 5' end
N = shift value for 3' end
Positive values shift the specified end in the 3' direction
Negative values shift the specified end in the 5' direction
Overlap selector definitions will be extended to optionally accept 2 numerical parameters. These will allow users to create rules that shift the 5'/3' ends of matching features by the specified number of bases.