tinyRNA provides an all-in-one solution for precision analysis of sRNA-seq data. At the core of tinyRNA is a highly flexible counting utility, tiny-count, that allows for hierarchical assignment of reads to features based on positional information, extent of feature overlap, 5’ nucleotide, length, and strandedness.
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tiny-count: make normalization by genomic hits optional #295
When tiny-count increments read counts for assigned features, the increment amount has been normalized twice from the sequence's original count:
1st by the number of genomic hits produced by the alignment tool for the sequence
2nd by the number of features selected at the sequence's given alignment locus.
tiny-count currently has the option to disable the 2nd normalization step, but not the first. It would be useful in some research contexts for normalization by genomic hits to be optional. Stats collection routines will also need to be updated to support the new option.
When tiny-count increments read counts for assigned features, the increment amount has been normalized twice from the sequence's original count:
tiny-count currently has the option to disable the 2nd normalization step, but not the first. It would be useful in some research contexts for normalization by genomic hits to be optional. Stats collection routines will also need to be updated to support the new option.