MontgomeryLab / tinyRNA

tinyRNA provides an all-in-one solution for precision analysis of sRNA-seq data. At the core of tinyRNA is a highly flexible counting utility, tiny-count, that allows for hierarchical assignment of reads to features based on positional information, extent of feature overlap, 5’ nucleotide, length, and strandedness.
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tiny-count: BAM support #304

Closed AlexTate closed 1 year ago

AlexTate commented 1 year ago

BAM files are now supported. This PR also improves the routine that examines alignment file headers to ensure that they have compatible order.

Additionally, the Samples Sheet is more thoroughly validated during tiny-count standalone runs. The Normalization column is also validated at pipeline startup during end-to-end runs

Closes #302

taimontgomery commented 1 year ago

Tested with Lib303 comparing sam and bam input files from tinyRNA output (collapsed or decollapsed). We might want to note in the documentation that the "Assigned Reads" reported depends on genomic and feature hit normalization.