If the sequence reads have a variable length according to FastQC (ex: ReadLength = 1-100), then the computation of the read depth fails. The PROJECT_quality_summary.txt output from Quality_Assessment has the number of reads listed again under the Read Depth column instead of a depth. This should be fixed to give depth estimates using the longest read length.
If the sequence reads have a variable length according to FastQC (ex: ReadLength = 1-100), then the computation of the read depth fails. The
PROJECT_quality_summary.txtoutput from Quality_Assessment has the number of reads listed again under the Read Depth column instead of a depth. This should be fixed to give depth estimates using the longest read length.