upper and lower LUT sliders completely overlap[BUG]

MouseLand / cellpose

a generalist algorithm for cellular segmentation with human-in-the-loop capabilities

https://www.cellpose.org/

BSD 3-Clause "New" or "Revised" License

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upper and lower LUT sliders completely overlap[BUG] #878

Closed derekthirstrup closed 3 months ago

derekthirstrup commented 4 months ago

Describe the bug The channel display LUT sliders overlap and can not be modified in many dim live cell images. Please add option to set values by actual pixel values and keep these settings for subsequent images.

Screenshots Here is an example to demonstrate the issue.

carsen-stringer commented 3 months ago

how many channels do you have in these images? is it fixed by turning off "auto-adjust"?

carsen-stringer commented 3 months ago

also please try pip install cellpose --upgrade, there was a bug in the saturation I thought I fixed

derekthirstrup commented 3 months ago

@carsen-stringer It appears to be fixed now in 3.0.6. I trained a label-free cell segmentation model so the image was a composite of brightfield and H2B endogenous EGFP channels. CP2 LUTs always needed to be adjusted depending on if H2B or the brightfield cell seg channels were being tested which is apparently fixed and each channel lower and upper LUT values now correctly get set.