I'm not sure what's going on, but for some cells my fluorescence signal is a flat line at 0. There is definitely fluorescence there. If I make the ROI super large so that it encompasses some regions outside the cell it will give me nonzero values, but that's not what I want -- I just want the cell.
I'm not sure what's going on, but for some cells my fluorescence signal is a flat line at 0. There is definitely fluorescence there. If I make the ROI super large so that it encompasses some regions outside the cell it will give me nonzero values, but that's not what I want -- I just want the cell.