I'm seeing an unexpected (to me) effect of sampling rate (fs) on segmentation. I'm using suite2p version 0.13.1, and running cellpose to get masks on max_proj/mean_imag.
Here is the mean image I get with approximately the real frame rate, 5.
Here is the max projection with the real frame rate:
And here is the mean image when I set fs to 10 times the real value (set to 50):
Here is the max projection:
What explains this behavior? Why does fs affect the max projections? Is there a different parameter I can change so that I can get the expected (higher) number of ROIs without using increasing fs?
I'm seeing an unexpected (to me) effect of sampling rate (fs) on segmentation. I'm using suite2p version 0.13.1, and running cellpose to get masks on max_proj/mean_imag.
Here is the mean image I get with approximately the real frame rate, 5.
Here is the max projection with the real frame rate:
And here is the mean image when I set fs to 10 times the real value (set to 50):
Here is the max projection:
What explains this behavior? Why does fs affect the max projections? Is there a different parameter I can change so that I can get the expected (higher) number of ROIs without using increasing fs?