Drep error with fastANI

[pengfeimm]

Hi Dr. Olm, Sorry for disturbing you, because I have installed fastANI but it is not working. Some error as below: [youfen.qian@compute-0-4]$ dRep check_dependencies mash.................................... all good (location = /share/data1/private/youfen.qian/software/miniconda3/envs/Compare/bin/mash) nucmer.................................. all good (location = /share/data1/private/youfen.qian/software/miniconda3/envs/Compare/bin/nucmer) checkm.................................. all good (location = /share/data1/private/youfen.qian/software/miniconda3/envs/Compare/bin/checkm) ANIcalculator........................... !!! ERROR !!! (location = None) prodigal................................ all good (location = /share/data1/private/youfen.qian/software/miniconda3/envs/Compare/bin/prodigal) centrifuge.............................. !!! ERROR !!! (location = None) nsimscan................................ !!! ERROR !!! (location = None) fastANI................................. !!! ERROR !!! (location = /share/data1/private/youfen.qian/software/miniconda3/envs/Compare/bin/fastANI)

File "/share/data1/private/youfen.qian/software/miniconda3/envs/Compare/lib/python3.7/site-packages/drep/d_cluster/compare_utils.py", line 298, in secondary_clustering ndb = compare_genomes(bdb, algorithm, data_folder, kwargs) File "/share/data1/private/youfen.qian/software/miniconda3/envs/Compare/lib/python3.7/site-packages/drep/d_cluster/compare_utils.py", line 368, in compare_genomes df = drep.d_cluster.external.run_pairwise_fastANI(genome_list, working_data_folder, kwargs) File "/share/data1/private/youfen.qian/software/miniconda3/envs/Compare/lib/python3.7/site-packages/drep/d_cluster/external.py", line 93, in run_pairwise_fastANI exe_loc = drep.get_exe('fastANI') File "/share/data1/private/youfen.qian/software/miniconda3/envs/Compare/lib/python3.7/site-packages/drep/init.py", line 100, in get_exe raise ValueError("{0} isn't working- make sure its installed".format(name)) ValueError: fastANI isn't working- make sure its installed

Can you give me some suggestion,Thanks. Pengfei.

[MrOlm]

Hi Pengfei,

This is a problem that can happen with older versions of fastANI. Please install the lastest version of fastANI and this problem should go away.

Best, Matt

[pengfeimm]

Thanks Matt， Problem solved well， Thanks again.