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NBISweden / workshop-scRNAseq

Single cell RNA sequencing analysis course
https://nbisweden.github.io/workshop-scRNAseq/
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Help #13

Closed CJSCI666 closed 2 years ago

CJSCI666 commented 3 years ago

Hello. I recently encountered a problem and I hope to get help. I didn’t understand the meaning of "PECAM1|PF4" very well when I ran this code. I tried to search for relevant information but did not find a satisfactory answer. I hope to get help. grateful!

Percentage hemoglobin genes - includes all genes starting with HB except HBP. alldata <- PercentageFeatureSet(alldata, "^HB[^(P)]", col.name = "percent_hb") alldata <- PercentageFeatureSet(alldata, "PECAM1|PF4", col.name = "percent_plat")

asabjorklund commented 2 years ago

Sorry @CJSCI666, for the late reply, PECAM1, PF4 are two platelet marker genes. So we are just trying to identify platelet cells by identifying droplets with high relative expression of those 2 genes.

CJSCI666 commented 2 years ago

您的信件我已经收到。 This is an automatic reply, confirming that your e-mail was received.Thank you