NCI-RBL / iCLIP

RNA Biology Pipeline to Characterize protein-RNA Interactions
https://rbl-nci.github.io/iCLIP/
MIT License
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limitOutSJcollapsed default value is too low for some samples #147

Closed slsevilla closed 1 year ago

slsevilla commented 1 year ago

Background: Yuanyuan encountered an error related to the --limitOutSJcollapsed in STAR

EXITING because of fatal error: buffer size for SJ output is too smallSolution: increase input parameter --limitOutSJcollapsed

looking at the manual, that flag is set by default:

--limitOutSJcollapsed
default: 1000000
int>0: max number of collapsed junctions

It's a known and documented issue: https://github.com/alexdobin/STAR/issues/719

Solution: 1) Update config file to include this as a flag 2) Update SNAKEMAKE, STAR RULE to include this flag 3) Publish new release with additional feature

slsevilla commented 1 year ago

added with commit b89dae8801134411b9d013515a11ec09094985ad