expand number of barcodes taken as input

[slsevilla]

change 02_barcode_qc.py script to include top 10 barcodes

marco ran into an error when inputting 6 barcodes, as max was set to 5 (unrelated to the wall time concern)

This particular dataset has 6 samples multiplexed. I got the following message:

The top barcodes identified ['GCCTAA', 'CGTGAT', 'TACAAG', 'TGGTCA', 'GATCTG'] were not congruent with expected barcode list ['CGTGAT', 'ACATCG', 'GCCTAA', 'TGGTCA', 'GATCTG', 'TACAAG']. Review associated img for more information.

The top 5 barcodes I got are from my barcode list, but it seems to be thrown off by the fact that there are six barcodes in my barcode list.

This was the associated error in the log files:

Activating environment modules: python/3.7 [-] Unloading snakemake 6.0.5 [+] Loading python 3.7 ... Waiting at most 120 seconds for missing files. MissingOutputException in line 385 of /data/RBL_NCI/Wolin/5-28-21_fCLIP_WT_Y5/workflow/Snakefile: Job Missing files after 120 seconds: /data/RBL_NCI/Wolin/5-28-21_fCLIP_WT_Y5/fCLIP/00_qc_post/fCLIP_barcode.txt This might be due to filesystem latency. If that is the case, consider to increase the wait time with --latency-wait. Job id: 0 completed successfully, but some output files are missing. 0 File "/usr/local/apps/snakemake/conda/envs/6.0.5/lib/python3.9/site-packages/snakemake/executors/init.py", line 583, in handle_job_success File "/usr/local/apps/snakemake/conda/envs/6.0.5/lib/python3.9/site-packages/snakemake/executors/init.py", line 252, in handle_job_success Removing output files of failed job qc_barcode since they might be corrupted: /data/RBL_NCI/Wolin/5-28-21_fCLIP_WT_Y5/fCLIP/00_qc_post/fCLIP_barcode.png Shutting down, this might take some time. Exiting because a job execution failed. Look above for error message

[slsevilla]

completed with commit d4aaa8a2fdae856dee1c14e7efb483d6a20152e0