NEUBIAS / training-resources

Resources for teaching/preparing to teach bioimage analysis
https://neubias.github.io/training-resources
Other
46 stars 21 forks source link

Object intensity measurements: Add background subtraction #281

Open tischi opened 2 years ago

tischi commented 2 years ago

@manerotoni @maulakhan

I think we should really add this somewhere:

Mean_Ratio = ( meanA - BG ) / (meanB - BG) Sum_Ratio = ( sumA - nPixelsA * BG ) / (sumB - nPixelsB * BG)

Where should we add it? I think we decided not to add it here and just use already BG-corrected images, but maybe that sends the wrong message.

We could rename: Global Background Correction to Background Measurement and Correction and then this module would be a prerequisite for Object intensity measurements.

What do you think?

manerotoni commented 2 years ago

Yes I think it belongs to Background assessment and correction. Would it make sense to reiterate the principle in the exercise with an image that is not background corrected. The students should spot the problem?

tischi commented 2 years ago

Would it make sense to reiterate the principle in the exercise with an image that is not background corrected.

To keep it simple I would then only do an image without background correction, because that's what you typically have.

tischi commented 2 years ago

I'll just try to this now to object intensity measurements (because I need to teach this on Tuesday). We can then refactor those modules at some other time.

tischi commented 2 years ago

Concept map looks like this now:

image

The module does become more complex, but I think that's life...