NUStatBioinfo / DegNorm

Normalizing RNA degradation in RNA-seq data
https://nustatbioinfo.github.io/DegNorm/
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Problem running DegNorm R package #43

Open paawanpoudel opened 3 years ago

paawanpoudel commented 3 years ago

Hi, I am trying to run Degnorm on ~19 samples (size of 1 bam file =11 GB) and the script gives me the following error message:

Loading required package: DegNorm Registered S3 method overwritten by 'seriation': method from reorder.hclust gclus Warning message: replacing previous import ‘vctrs::data_frame’ by ‘tibble::data_frame’ when loading ‘dplyr’ Error in unserialize(socklist[[n]]) : error reading from connection Calls: ... recvOneData -> recvOneData.SOCKcluster -> unserialize Execution halted

Here is the command I used:

estimating the gene specific coverage

coverage_res <- DegNorm::read_coverage_batch( bam_files, gtf_file, cores = n_cores )

Any comments from you would be really helpful.