Closed r-barnes closed 4 years ago
Here's the fastq: https://www.ncbi.nlm.nih.gov/sra/SRX145461
Added documentation to README. Thanks!
@rdwrt: I wasn't able to get that file to work. Do you have a more specific link (this one brings you to a webpage from which there seem to be several files)?
you have to first build the .bwt from either a single fasta file or the complete list chromosomes, using nvBWT: https://nvlabs.github.io/nvbio/nvbwt_page.html
A single file for hg18 can be found here: ftp://genome-ftp.cse.ucsc.edu/goldenPath/hg18/bigZips/hg18.2bit
You have to use a utility twoBitToFa to convert it to fasta (though maybe we have also added support for reading directly from it, I don't recall).
Alternatively, you can find all chromosomes for hg18 here: http://hgdownload.cse.ucsc.edu/goldenpath/hg18/chromosomes/
Or you could use the top-level assembly for hg19 here: ftp://ftp.ensembl.org/pub/grch37/current/fasta/homo_sapiens/dna/
But I'd go for the .2bit file first, easiest and less error prone since it's already assembled.
On Thu, Feb 6, 2020 at 2:16 AM Richard Barnes notifications@github.com wrote:
@rdwrt https://github.com/rdwrt: I wasn't able to get that file to work. Do you have a more specific link (this one brings you to a webpage from which there seem to be several files)?
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Running
./nvbio-test/nvbio-test
gives the following errors:These files were likely too large to include in the repo directory, but prevent the test suite from completing. Is there a way to get them uploaded somewhere?