Wild zoea micro-disection

NWFSC-OA-lab / dng_zoe_exo_skl_2021

0 stars 0 forks source link

Wild zoea micro-disection #8

Open pmcelhany opened 3 years ago

pmcelhany commented 3 years ago

[x] Collect preserved wild zoea for practice micro-dissection
[x] Buy Micro-injection plates
[x] Buy Fine forceps
[x] Buy agar
[x] download and install IC capture
[x] Research stovetop agar
[ ] practice micro-dissection (Gagnon lab)
[ ] Write sop (with pictures) of dissection method

dmperez89 commented 3 years ago

While still not knowing too much about the details of how to micro-dissect, these are apparently the most popular plate mold, so they might work for us. I also had no idea how expensive fine forceps were! I'm not sure just how fine we need to go, but 0.3 x 0.15mm or 0.2 x 0.12mm seem pretty fine without costing too terribly much. Any thoughts?

pmcelhany commented 3 years ago

Sure. Let's try the plate mold and one of each of those sizes of forceps. We need two forceps to pull things apart and if we get one of each you can see which size you like and we can get another if needed. I think Kathleen is out of town, so Mike will need to order them.

michaelmaher114 commented 3 years ago

Danielle is going to call Mike

pmcelhany commented 2 years ago

@dmperez89 Can you add scale bars to the photos of dissected crab bits?