Open mgrout81 opened 11 months ago
I am using mu_dev_E9_003 in SOO and trying to run plotSpatialOverlay() with fluorLegend=TRUE. I get this error:
Error in `[[<-.data.frame`(`*tmp*`, i, value = c(DNA = "#0000FF", Custom = "#00FFFF", : replacement has 4 rows, data has 2
I believe what is likely happening is that the code is assuming unique Target values for all rows, but I only have 2, "DNA", and "Custom":
> fluor(overlay) Dye DisplayName Color WaveLength Target ExposureTime MinIntensity MaxIntensity ColorCode 1 SYTO 13 FITC Blue 525nm DNA 100.0 µs 0 3000 #0000FF 2 Alexa 532 Cy3 Cyan 568nm Custom 300.0 µs 0 1500 #00FFFF 3 Alexa 594 Texas Red Green 615nm Custom 300.0 µs 0 975 #00FF00 4 Cy5 Cy5 Deeppink2 666nm Custom 300.0 µs 350 2500 #FF0054
https://github.com/Nanostring-Biostats/SpatialOmicsOverlay/blob/a78d1bb386ff16daa3f83de907e6e7868c4bb9f0/R/plottingBasics.R#L129
I assume "Custom" indicates a lack of something in particular specified by the user on the DSP instrument, so likely this is something that will not be too uncommon.
I am using mu_dev_E9_003 in SOO and trying to run plotSpatialOverlay() with fluorLegend=TRUE. I get this error:
I believe what is likely happening is that the code is assuming unique Target values for all rows, but I only have 2, "DNA", and "Custom":
https://github.com/Nanostring-Biostats/SpatialOmicsOverlay/blob/a78d1bb386ff16daa3f83de907e6e7868c4bb9f0/R/plottingBasics.R#L129
I assume "Custom" indicates a lack of something in particular specified by the user on the DSP instrument, so likely this is something that will not be too uncommon.