Work on learning skyline

[NatalieDreux]

4/15/2024

Melissa finally ran my samples on the LUMOS, now I need to figure out the next steps in processing the data.

Initial Thoughts

• I need to convert the data into the correct format
• Use the SkyMat help guide in the KujLab GitHub to import data to skyline
• Start small to get comfortable
• Meeting with Yuting on Thurs. (4/18) for questions and comments ... working on it alone on Wednesday to understand my gaps

[NatalieDreux]

Began integrating the peaking in ALL samples for the top 5 metabolites based on Yuting exometabolite data.

[NatalieDreux]

All peaks for top 5 metabolites have been integrated for all samples ran in the Pro11 MIT samples.

1. Alanine
2. Aspartate
3. Glutamic acid
4. Isoleucine
5. Valine

The data has been exported into a .csv file and filtered in order to only keep the data from the metabolites where the peaks were inegrated. This .csv along with the given sequence and transition list will be uploaded into to SkyMat system created in the Kuj lab to analyze the metabolite information to get initial results.

Pro11BC_TopFive_Neg_NDG_skylinedata.csv