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NelleV / moanin

Timecourse transcriptomic analysis
https://nellev.github.io/moanin/
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how to create contrasts for a very simple experiment design? #98

Open eterlova opened 1 year ago

eterlova commented 1 year ago

Hello,

I am trying to analyze a time-course data for just one genotype, i.e. my metadata looks like this: Condition SampleName Timepoint physiological condition 1 sample1 1 physiological condition 2 sample2 2 physiological condition 1 sample3 1 ...

I succeeded in creating a moaninObject, but when I run create_timepoints_contrasts (... type="per_group_timepoint_diff") I get this error

Error in .which_timepoints(timepoints_before, all_timepoints, "timepoints_before") : 
  None of the requested timepoints measured for these groups

I am guessing that I don't understand what should I use as a Group1 argument here. Could anybody help?

Best, Lisa