The "Quan usage" implications in the TMT-10 workflow processing PSM.tsv results

[HYCCHENG]

• Describe the issue or question:

Hi,We have recently used the TMT workflow for quantitative analysis of data post-translational modification sites, and in the PSM.tsv results， there is a column "quan usage" that does not give a specific meaning in the operating guide. What does this information mean? Thanks.

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[fcyu]

It means that if that PSM is used in the TMT intensity calculation.

Best,

Fengchao

[HYCCHENG]

thanks！Another question is If the “quan usage” output is false and the TMT channel still gives the intensity value, on what basis is this value given？

[fcyu]

The intensity is from another PSM of the same peptide/protein/site.

Best,

Fengchao

[HYCCHENG]

thank you~~