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Nesvilab / FragPipe

A cross-platform proteomics data analysis suite
http://fragpipe.nesvilab.org
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Incompatible options: MSFragger-DIA split database is incompatible with DIA, GPF-DIA, DIA-Lib, or DDA+ data types #1633

Closed helloworld1631 closed 3 months ago

helloworld1631 commented 3 months ago

Hi Fengchao,

Following your suggestion, I tried to search my semi-specific cleaved DIA raw files using the DIA_DIA-Umpire_SpecLib_Quant workflow. However, due to the large search space, I need to split the database to fit my PC memory capacity.

However, after clicking Run, an error message immediately appeared (as shown in the image), despite confirming that I loaded the DIA_DIA-Umpire_SpecLib_Quant workflow. Could you advise on the correct way to use the Split Database function in DIA searching mode? image

Thank you! Zeyu

fcyu commented 3 months ago

Could you advise on the correct way to use the Split Database function in DIA searching mode?

It is not supported well.

Best,

Fengchao

helloworld1631 commented 3 months ago

I see. Will this be supported in the future?

Thanks, Zeyu

fcyu commented 3 months ago

Yes.