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Nesvilab / FragPipe

A cross-platform proteomics data analysis suite
http://fragpipe.nesvilab.org
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No Ms1 scans detected in LFQ-MBR #1774

Closed aarondemingg closed 4 weeks ago

aarondemingg commented 4 weeks ago

Dear Fragpipe team,

I have encountered an issue with the LFQ-MBR workflow. To start with, I converted my (.mgf) files into mzML with MSconvert and ran the default LFQ-MBR workflow (DDA datatype). I tried numerous ways in the conversion step to solve the issue but was unable to do so. Would appreciate it greatly if anyone could help me out. I have attached the log below. Thanks!

log_2024-09-08_21-55-17.txt

fcyu commented 4 weeks ago

I converted my (.mgf) files into mzML with MSconvert and ran the default LFQ-MBR workflow

MGF files don't have MS1 scans. Please use the raw format files or the mzML files converted from the raw.

Best,

Fengchao

aarondemingg commented 4 weeks ago

Dear FengChao,

Ahh. Thank you so much!

cheers, Aaron