fcyu
commented
2 weeks ago Same as https://github.com/Nesvilab/FragPipe/issues/1836, please follow intructions from https://github.com/Nesvilab/FragPipe/issues/1836#issuecomment-2430217130 to resolve.
Best,
Fengchao
log_2024-11-14_11-26-09.txt
I am trying to run DIA-NN through FragPipe on Astral DIA raw files with the DIA_SpecLib_Quant workflow. I have used Proteowizard to create 32bit mzml files with vendor peak picking for centroiding.
However, I get the following error message (see log):
ImportError: DLL load failed while importing _pyopenms_1: A dynamic link library (DLL) initialization routine failed.
====================================================================== Error when loading pyOpenMS libraries! Libraries could not be found / could not be loaded.
To debug this error, please run ldd (on linux), otool -L (on macOS) or dependency walker (on windows) on
C:\Users\maxquant\anaconda3\Lib\site-packages\pyopenms\pyopenms*.so
====================================================================== PyQt5 was found to be installed. When building pyopenms qmake said:
PYQT has version 5.15.7
This might cause a conflict if both are loaded. You can test this by importing pyopenms first and then import PyQt5.QtCore.
Note: when using the Spyder IDE, the usage of PyQt might be circumvented by not using the 'Automatic' backend. Please change this in Tools -> Preferences -> IPython -> Graphics to 'Inline'. In general, try to install everything with conda in the same environment to make sure Qt is used in the same version.
======================================================================