Closed rajeshksoni11 closed 2 years ago
Thanks for your report. Can you upload your .d folders, fasta file, and fragger.params to https://www.dropbox.com/request/blovHST1fE9mOvuMM13x ?
Best,
Fengchao
On Thu, Aug 5, 2021 at 11:31 AM rajeshksoni11 @.***> wrote:
-
Describe the log_2021-08-05_11-29-12.txt https://github.com/Nesvilab/FragPipe/files/6939813/log_2021-08-05_11-29-12.txt issue or question:
Upload your log file (If a log file hasn't been generated, go to the 'Run' tab in FragPipe, click 'Export Log', zip the resulting "log_[date_time].txt" file to avoid truncation, then attach the zipped file by drag & drop here.)
— You are receiving this because you are subscribed to this thread. Reply to this email directly, view it on GitHub https://github.com/Nesvilab/FragPipe/issues/419, or unsubscribe https://github.com/notifications/unsubscribe-auth/ABU27W4E2LANBTNISTH2GS3T3KVDZANCNFSM5BUDBJPA . Triage notifications on the go with GitHub Mobile for iOS https://apps.apple.com/app/apple-store/id1477376905?ct=notification-email&mt=8&pt=524675 or Android https://play.google.com/store/apps/details?id=com.github.android&utm_campaign=notification-email .
-- Dr. Fengchao Yu Research Investigator University of Michigan
Hi all, Thanks. I am uploading the folder, and another issue, java.lang.OutOfMemoryError, all the time if I search for a non-specific HLA search. I have 64GB ram and selected Ram= o(auto) and parallism= 10 Please suggest I fix the issues. best, Rajesh
From: Fengchao @.> Sent: Thursday, August 5, 2021 11:35 AM To: Nesvilab/FragPipe @.> Cc: Soni, Rajesh K. @.>; Author @.> Subject: [EXTERNAL] Re: [Nesvilab/FragPipe] Stopped (#419)
Thanks for your report. Can you upload your .d folders, fasta file, and fragger.params to https://www.dropbox.com/request/blovHST1fE9mOvuMM13x ?
Best,
Fengchao
On Thu, Aug 5, 2021 at 11:31 AM rajeshksoni11 @.***> wrote:
-
Describe the log_2021-08-05_11-29-12.txt https://github.com/Nesvilab/FragPipe/files/6939813/log_2021-08-05_11-29-12.txt issue or question:
Upload your log file (If a log file hasn't been generated, go to the 'Run' tab in FragPipe, click 'Export Log', zip the resulting "log_[date_time].txt" file to avoid truncation, then attach the zipped file by drag & drop here.)
— You are receiving this because you are subscribed to this thread. Reply to this email directly, view it on GitHub https://github.com/Nesvilab/FragPipe/issues/419, or unsubscribe https://github.com/notifications/unsubscribe-auth/ABU27W4E2LANBTNISTH2GS3T3KVDZANCNFSM5BUDBJPA . Triage notifications on the go with GitHub Mobile for iOS https://apps.apple.com/app/apple-store/id1477376905?ct=notification-email&mt=8&pt=524675 or Android https://play.google.com/store/apps/details?id=com.github.android&utm_campaign=notification-email .
-- Dr. Fengchao Yu Research Investigator University of Michigan
— You are receiving this because you authored the thread. Reply to this email directly, view it on GitHubhttps://urldefense.proofpoint.com/v2/url?u=https-3A__github.com_Nesvilab_FragPipe_issues_419-23issuecomment-2D893557340&d=DwMFaQ&c=G2MiLlal7SXE3PeSnG8W6_JBU6FcdVjSsBSbw6gcR0U&r=3kGEWBYAR_TOTPC4fFAqpAKTOUJHGP7jqdAzaZ1xUoQ&m=Vv0AUnJce_Zi3awlNhV5ACiBnIKzikq6DMceZ79QY08&s=pnm408QC5BIiqwIlqIoJnyMpBncliQYPXkALvd16iRY&e=, or unsubscribehttps://urldefense.proofpoint.com/v2/url?u=https-3A__github.com_notifications_unsubscribe-2Dauth_ATHFHTGWGKIGIQSIB7WCRKDT3KVVHANCNFSM5BUDBJPA&d=DwMFaQ&c=G2MiLlal7SXE3PeSnG8W6_JBU6FcdVjSsBSbw6gcR0U&r=3kGEWBYAR_TOTPC4fFAqpAKTOUJHGP7jqdAzaZ1xUoQ&m=Vv0AUnJce_Zi3awlNhV5ACiBnIKzikq6DMceZ79QY08&s=W6-VlQ5Yucdg7dnaR-KX_xw8oMFxkAFbcavEaRZjJQQ&e=. Triage notifications on the go with GitHub Mobile for iOShttps://urldefense.proofpoint.com/v2/url?u=https-3A__apps.apple.com_app_apple-2Dstore_id1477376905-3Fct-3Dnotification-2Demail-26mt-3D8-26pt-3D524675&d=DwMFaQ&c=G2MiLlal7SXE3PeSnG8W6_JBU6FcdVjSsBSbw6gcR0U&r=3kGEWBYAR_TOTPC4fFAqpAKTOUJHGP7jqdAzaZ1xUoQ&m=Vv0AUnJce_Zi3awlNhV5ACiBnIKzikq6DMceZ79QY08&s=Od4ibP_O7dmvvggzj3Xgncs5XHsMlKT8BgMJ4yUyzP4&e= or Androidhttps://urldefense.proofpoint.com/v2/url?u=https-3A__play.google.com_store_apps_details-3Fid-3Dcom.github.android-26utm-5Fcampaign-3Dnotification-2Demail&d=DwMFaQ&c=G2MiLlal7SXE3PeSnG8W6_JBU6FcdVjSsBSbw6gcR0U&r=3kGEWBYAR_TOTPC4fFAqpAKTOUJHGP7jqdAzaZ1xUoQ&m=Vv0AUnJce_Zi3awlNhV5ACiBnIKzikq6DMceZ79QY08&s=1-Uzcfqb8mZFWbpJsq-pfHZr_9qea_bUhMK4NclEjFg&e=.
Hi Rajesh,
Non-specific search requires lots of memory. If 64 GB is not enough for your data, you can increase the value for "split database" in MSFragger tab (the default value is 1 meaning no splitting).
Best,
Fengchao
On Thu, Aug 5, 2021 at 1:05 PM rajeshksoni11 @.***> wrote:
Hi all, Thanks. I am uploading the folder, and another issue, java.lang.OutOfMemoryError, all the time if I search for a non-specific HLA search. I have 64GB ram and selected Ram= o(auto) and parallism= 10 Please suggest I fix the issues. best, Rajesh
From: Fengchao @.> Sent: Thursday, August 5, 2021 11:35 AM To: Nesvilab/FragPipe @.> Cc: Soni, Rajesh K. @.>; Author @.> Subject: [EXTERNAL] Re: [Nesvilab/FragPipe] Stopped (#419)
Thanks for your report. Can you upload your .d folders, fasta file, and fragger.params to https://www.dropbox.com/request/blovHST1fE9mOvuMM13x ?
Best,
Fengchao
On Thu, Aug 5, 2021 at 11:31 AM rajeshksoni11 @.***> wrote:
-
Describe the log_2021-08-05_11-29-12.txt < https://github.com/Nesvilab/FragPipe/files/6939813/log_2021-08-05_11-29-12.txt
issue or question:
Upload your log file (If a log file hasn't been generated, go to the 'Run' tab in FragPipe, click 'Export Log', zip the resulting "log_[date_time].txt" file to avoid truncation, then attach the zipped file by drag & drop here.)
— You are receiving this because you are subscribed to this thread. Reply to this email directly, view it on GitHub https://github.com/Nesvilab/FragPipe/issues/419, or unsubscribe < https://github.com/notifications/unsubscribe-auth/ABU27W4E2LANBTNISTH2GS3T3KVDZANCNFSM5BUDBJPA
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or Android < https://play.google.com/store/apps/details?id=com.github.android&utm_campaign=notification-email
.
-- Dr. Fengchao Yu Research Investigator University of Michigan
— You are receiving this because you authored the thread. Reply to this email directly, view it on GitHub< https://urldefense.proofpoint.com/v2/url?u=https-3A__github.com_Nesvilab_FragPipe_issues_419-23issuecomment-2D893557340&d=DwMFaQ&c=G2MiLlal7SXE3PeSnG8W6_JBU6FcdVjSsBSbw6gcR0U&r=3kGEWBYAR_TOTPC4fFAqpAKTOUJHGP7jqdAzaZ1xUoQ&m=Vv0AUnJce_Zi3awlNhV5ACiBnIKzikq6DMceZ79QY08&s=pnm408QC5BIiqwIlqIoJnyMpBncliQYPXkALvd16iRY&e=>, or unsubscribe< https://urldefense.proofpoint.com/v2/url?u=https-3A__github.com_notifications_unsubscribe-2Dauth_ATHFHTGWGKIGIQSIB7WCRKDT3KVVHANCNFSM5BUDBJPA&d=DwMFaQ&c=G2MiLlal7SXE3PeSnG8W6_JBU6FcdVjSsBSbw6gcR0U&r=3kGEWBYAR_TOTPC4fFAqpAKTOUJHGP7jqdAzaZ1xUoQ&m=Vv0AUnJce_Zi3awlNhV5ACiBnIKzikq6DMceZ79QY08&s=W6-VlQ5Yucdg7dnaR-KX_xw8oMFxkAFbcavEaRZjJQQ&e=
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-- Dr. Fengchao Yu Research Investigator University of Michigan
Hi Fengcho, I am using the UniProt mouse database, how much value I should keep for spilt database? Best, Rajesh
Rajesh Kumar Soni , PhD Proteomics Core Manager Lasker Biomedical Research Building 3960 Broadway, 3rd Floor, Room 350D (Entrance on 166th St.) Columbia University Medical Center New York, NY 10032 Email: @.*** Tel: 212-342-4177
From: Fengchao @.> Sent: Thursday, August 5, 2021 1:09 PM To: Nesvilab/FragPipe @.> Cc: Soni, Rajesh K. @.>; Author @.> Subject: [EXTERNAL] Re: [Nesvilab/FragPipe] Stopped (#419)
Hi Rajesh,
Non-specific search requires lots of memory. If 64 GB is not enough for your data, you can increase the value for "split database" in MSFragger tab (the default value is 1 meaning no splitting).
Best,
Fengchao
On Thu, Aug 5, 2021 at 1:05 PM rajeshksoni11 @.***> wrote:
Hi all, Thanks. I am uploading the folder, and another issue, java.lang.OutOfMemoryError, all the time if I search for a non-specific HLA search. I have 64GB ram and selected Ram= o(auto) and parallism= 10 Please suggest I fix the issues. best, Rajesh
From: Fengchao @.> Sent: Thursday, August 5, 2021 11:35 AM To: Nesvilab/FragPipe @.> Cc: Soni, Rajesh K. @.>; Author @.> Subject: [EXTERNAL] Re: [Nesvilab/FragPipe] Stopped (#419)
Thanks for your report. Can you upload your .d folders, fasta file, and fragger.params to https://www.dropbox.com/request/blovHST1fE9mOvuMM13x ?
Best,
Fengchao
On Thu, Aug 5, 2021 at 11:31 AM rajeshksoni11 @.***> wrote:
-
Describe the log_2021-08-05_11-29-12.txt < https://github.com/Nesvilab/FragPipe/files/6939813/log_2021-08-05_11-29-12.txt
issue or question:
Upload your log file (If a log file hasn't been generated, go to the 'Run' tab in FragPipe, click 'Export Log', zip the resulting "log_[date_time].txt" file to avoid truncation, then attach the zipped file by drag & drop here.)
— You are receiving this because you are subscribed to this thread. Reply to this email directly, view it on GitHub https://github.com/Nesvilab/FragPipe/issues/419, or unsubscribe < https://github.com/notifications/unsubscribe-auth/ABU27W4E2LANBTNISTH2GS3T3KVDZANCNFSM5BUDBJPA
. Triage notifications on the go with GitHub Mobile for iOS < https://apps.apple.com/app/apple-store/id1477376905?ct=notification-email&mt=8&pt=524675
or Android < https://play.google.com/store/apps/details?id=com.github.android&utm_campaign=notification-email
.
-- Dr. Fengchao Yu Research Investigator University of Michigan
— You are receiving this because you authored the thread. Reply to this email directly, view it on GitHub< https://urldefense.proofpoint.com/v2/url?u=https-3A__github.com_Nesvilab_FragPipe_issues_419-23issuecomment-2D893557340&d=DwMFaQ&c=G2MiLlal7SXE3PeSnG8W6_JBU6FcdVjSsBSbw6gcR0U&r=3kGEWBYAR_TOTPC4fFAqpAKTOUJHGP7jqdAzaZ1xUoQ&m=Vv0AUnJce_Zi3awlNhV5ACiBnIKzikq6DMceZ79QY08&s=pnm408QC5BIiqwIlqIoJnyMpBncliQYPXkALvd16iRY&e=>, or unsubscribe< https://urldefense.proofpoint.com/v2/url?u=https-3A__github.com_notifications_unsubscribe-2Dauth_ATHFHTGWGKIGIQSIB7WCRKDT3KVVHANCNFSM5BUDBJPA&d=DwMFaQ&c=G2MiLlal7SXE3PeSnG8W6_JBU6FcdVjSsBSbw6gcR0U&r=3kGEWBYAR_TOTPC4fFAqpAKTOUJHGP7jqdAzaZ1xUoQ&m=Vv0AUnJce_Zi3awlNhV5ACiBnIKzikq6DMceZ79QY08&s=W6-VlQ5Yucdg7dnaR-KX_xw8oMFxkAFbcavEaRZjJQQ&e=
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-- Dr. Fengchao Yu Research Investigator University of Michigan
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It's hard to tell because it depends on your variable modifications etc. You can try different values from small to large.
Best,
Fengchao
On Thu, Aug 5, 2021 at 1:19 PM rajeshksoni11 @.***> wrote:
Hi Fengcho, I am using the UniProt mouse database, how much value I should keep for spilt database? Best, Rajesh
Rajesh Kumar Soni , PhD Proteomics Core Manager Lasker Biomedical Research Building 3960 Broadway, 3rd Floor, Room 350D (Entrance on 166th St.) Columbia University Medical Center New York, NY 10032 Email: @.*** Tel: 212-342-4177
From: Fengchao @.> Sent: Thursday, August 5, 2021 1:09 PM To: Nesvilab/FragPipe @.> Cc: Soni, Rajesh K. @.>; Author @.> Subject: [EXTERNAL] Re: [Nesvilab/FragPipe] Stopped (#419)
Hi Rajesh,
Non-specific search requires lots of memory. If 64 GB is not enough for your data, you can increase the value for "split database" in MSFragger tab (the default value is 1 meaning no splitting).
Best,
Fengchao
On Thu, Aug 5, 2021 at 1:05 PM rajeshksoni11 @.***> wrote:
Hi all, Thanks. I am uploading the folder, and another issue, java.lang.OutOfMemoryError, all the time if I search for a non-specific HLA search. I have 64GB ram and selected Ram= o(auto) and parallism= 10 Please suggest I fix the issues. best, Rajesh
From: Fengchao @.> Sent: Thursday, August 5, 2021 11:35 AM To: Nesvilab/FragPipe @.> Cc: Soni, Rajesh K. @.>; Author @.> Subject: [EXTERNAL] Re: [Nesvilab/FragPipe] Stopped (#419)
Thanks for your report. Can you upload your .d folders, fasta file, and fragger.params to https://www.dropbox.com/request/blovHST1fE9mOvuMM13x ?
Best,
Fengchao
On Thu, Aug 5, 2021 at 11:31 AM rajeshksoni11 @.***> wrote:
-
Describe the log_2021-08-05_11-29-12.txt <
https://github.com/Nesvilab/FragPipe/files/6939813/log_2021-08-05_11-29-12.txt
issue or question:
Upload your log file (If a log file hasn't been generated, go to the 'Run' tab in FragPipe, click 'Export Log', zip the resulting "log_[date_time].txt" file to avoid truncation, then attach the zipped file by drag & drop here.)
— You are receiving this because you are subscribed to this thread. Reply to this email directly, view it on GitHub https://github.com/Nesvilab/FragPipe/issues/419, or unsubscribe <
https://github.com/notifications/unsubscribe-auth/ABU27W4E2LANBTNISTH2GS3T3KVDZANCNFSM5BUDBJPA
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or Android <
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.
-- Dr. Fengchao Yu Research Investigator University of Michigan
— You are receiving this because you authored the thread. Reply to this email directly, view it on GitHub<
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-- Dr. Fengchao Yu Research Investigator University of Michigan
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-- Dr. Fengchao Yu Research Investigator University of Michigan
Hi Rajesh,
Thanks for your uploaded files. It took me a while to figure out the reason. It is due to a bug/feature in Philosopher, and another bug/feature in univocity-parsers
which is the library used by IonQuant to parse tsv files. Regarding the univocity-parsers
one, I have submitted an issue to https://github.com/uniVocity/univocity-parsers/issues/472
Regarding Philosopher, there is no ion mobility
column in psm.tsv
from HD_PASEF_PyR1_Ingeldig_Mdx_Trypsin_Slot2-3_1_1361
. Thus, there are two different column sets in two psm.tsv files:
The first psm.tsv file
The second psm.tsv file
It triggers the bug/feature in univocity-parsers
. Since our first run has no PSM passing FDR threshold, you can remove that run for now.
@prvst Can you change Philosopher to let it still print ion mobility
column if there is no PSM but the data is from ddaPASEF? I guess the same idea should also applied to FAIMS' compensation voltage
column. Not sure about TMT, though.
Thanks,
Fengchao
Hi Fengchao, Thanks. How to get the STY table in output? I am using Timstofpro data. Best, Rajesh
Rajesh Kumar Soni , PhD Proteomics Core Manager Lasker Biomedical Research Building 3960 Broadway, 3rd Floor, Room 350D (Entrance on 166th St.) Columbia University Medical Center New York, NY 10032 Email: @.*** Tel: 212-342-4177
From: Fengchao @.> Sent: Thursday, August 5, 2021 1:21 PM To: Nesvilab/FragPipe @.> Cc: Soni, Rajesh K. @.>; Author @.> Subject: [EXTERNAL] Re: [Nesvilab/FragPipe] Stopped (#419)
It's hard to tell because it depends on your variable modifications etc. You can try different values from small to large.
Best,
Fengchao
On Thu, Aug 5, 2021 at 1:19 PM rajeshksoni11 @.***> wrote:
Hi Fengcho, I am using the UniProt mouse database, how much value I should keep for spilt database? Best, Rajesh
Rajesh Kumar Soni , PhD Proteomics Core Manager Lasker Biomedical Research Building 3960 Broadway, 3rd Floor, Room 350D (Entrance on 166th St.) Columbia University Medical Center New York, NY 10032 Email: @.*** Tel: 212-342-4177
From: Fengchao @.> Sent: Thursday, August 5, 2021 1:09 PM To: Nesvilab/FragPipe @.> Cc: Soni, Rajesh K. @.>; Author @.> Subject: [EXTERNAL] Re: [Nesvilab/FragPipe] Stopped (#419)
Hi Rajesh,
Non-specific search requires lots of memory. If 64 GB is not enough for your data, you can increase the value for "split database" in MSFragger tab (the default value is 1 meaning no splitting).
Best,
Fengchao
On Thu, Aug 5, 2021 at 1:05 PM rajeshksoni11 @.***> wrote:
Hi all, Thanks. I am uploading the folder, and another issue, java.lang.OutOfMemoryError, all the time if I search for a non-specific HLA search. I have 64GB ram and selected Ram= o(auto) and parallism= 10 Please suggest I fix the issues. best, Rajesh
From: Fengchao @.> Sent: Thursday, August 5, 2021 11:35 AM To: Nesvilab/FragPipe @.> Cc: Soni, Rajesh K. @.>; Author @.> Subject: [EXTERNAL] Re: [Nesvilab/FragPipe] Stopped (#419)
Thanks for your report. Can you upload your .d folders, fasta file, and fragger.params to https://www.dropbox.com/request/blovHST1fE9mOvuMM13x ?
Best,
Fengchao
On Thu, Aug 5, 2021 at 11:31 AM rajeshksoni11 @.***> wrote:
-
Describe the log_2021-08-05_11-29-12.txt <
https://github.com/Nesvilab/FragPipe/files/6939813/log_2021-08-05_11-29-12.txt
issue or question:
Upload your log file (If a log file hasn't been generated, go to the 'Run' tab in FragPipe, click 'Export Log', zip the resulting "log_[date_time].txt" file to avoid truncation, then attach the zipped file by drag & drop here.)
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Hi Rajesh,
Do you mean the phospho site table? We don’t have it for LFQ, but we are working on it. Should be available in the next version.
Best,
Fengchao
On Sun, 15 Aug 2021 at 5:34 PM, rajeshksoni11 @.***> wrote:
Hi Fengchao, Thanks. How to get the STY table in output? I am using Timstofpro data. Best, Rajesh
Rajesh Kumar Soni , PhD Proteomics Core Manager Lasker Biomedical Research Building 3960 Broadway, 3rd Floor, Room 350D https://www.google.com/maps/search/3960+Broadway,+3rd+Floor,+Room+350D?entry=gmail&source=g (Entrance on 166th St.) Columbia University Medical Center New York, NY 10032 Email: @.*** Tel: 212-342-4177
From: Fengchao @.> Sent: Thursday, August 5, 2021 1:21 PM To: Nesvilab/FragPipe @.> Cc: Soni, Rajesh K. @.>; Author @.> Subject: [EXTERNAL] Re: [Nesvilab/FragPipe] Stopped (#419)
It's hard to tell because it depends on your variable modifications etc. You can try different values from small to large.
Best,
Fengchao
On Thu, Aug 5, 2021 at 1:19 PM rajeshksoni11 @.***> wrote:
Hi Fengcho, I am using the UniProt mouse database, how much value I should keep for spilt database? Best, Rajesh
Rajesh Kumar Soni , PhD Proteomics Core Manager Lasker Biomedical Research Building 3960 Broadway, 3rd Floor, Room 350D https://www.google.com/maps/search/3960+Broadway,+3rd+Floor,+Room+350D?entry=gmail&source=g (Entrance on 166th St.) Columbia University Medical Center New York, NY 10032 Email: @.*** Tel: 212-342-4177
From: Fengchao @.> Sent: Thursday, August 5, 2021 1:09 PM To: Nesvilab/FragPipe @.> Cc: Soni, Rajesh K. @.>; Author @.> Subject: [EXTERNAL] Re: [Nesvilab/FragPipe] Stopped (#419)
Hi Rajesh,
Non-specific search requires lots of memory. If 64 GB is not enough for your data, you can increase the value for "split database" in MSFragger tab (the default value is 1 meaning no splitting).
Best,
Fengchao
On Thu, Aug 5, 2021 at 1:05 PM rajeshksoni11 @.***> wrote:
Hi all, Thanks. I am uploading the folder, and another issue, java.lang.OutOfMemoryError, all the time if I search for a non-specific HLA search. I have 64GB ram and selected Ram= o(auto) and parallism= 10 Please suggest I fix the issues. best, Rajesh
From: Fengchao @.> Sent: Thursday, August 5, 2021 11:35 AM To: Nesvilab/FragPipe @.> Cc: Soni, Rajesh K. @.>; Author @.> Subject: [EXTERNAL] Re: [Nesvilab/FragPipe] Stopped (#419)
Thanks for your report. Can you upload your .d folders, fasta file, and fragger.params to https://www.dropbox.com/request/blovHST1fE9mOvuMM13x ?
Best,
Fengchao
On Thu, Aug 5, 2021 at 11:31 AM rajeshksoni11 @.***> wrote:
-
Describe the log_2021-08-05_11-29-12.txt <
https://github.com/Nesvilab/FragPipe/files/6939813/log_2021-08-05_11-29-12.txt
issue or question:
Upload your log file (If a log file hasn't been generated, go to the 'Run' tab in FragPipe, click 'Export Log', zip the resulting "log_[date_time].txt" file to avoid truncation, then attach the zipped file by drag & drop here.)
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-- Dr. Fengchao Yu Research Investigator University of Michigan
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-- Dr. Fengchao Yu Research Investigator University of Michigan
Hi Fengchao, Thank you for your quick response. Yes, i am very interested in phosho-sites table with LFQ intensities. Best, Rajesh
Rajesh Kumar Soni , PhD Proteomics Core Manager Lasker Biomedical Research Building 3960 Broadway, 3rd Floor, Room 350D (Entrance on 166th St.) Columbia University Medical Center New York, NY 10032 Email: @.*** Tel: 212-342-4177
From: Fengchao @.> Sent: Sunday, August 15, 2021 5:36 PM To: Nesvilab/FragPipe @.> Cc: Soni, Rajesh K. @.>; Author @.> Subject: [EXTERNAL] Re: [Nesvilab/FragPipe] Philosopher does not print 'ion mobility' column to psm.tsv if there is no PSM but the data is from ddaPASEF (#419)
Hi Rajesh,
Do you mean the phospho site table? We don’t have it for LFQ, but we are working on it. Should be available in the next version.
Best,
Fengchao
On Sun, 15 Aug 2021 at 5:34 PM, rajeshksoni11 @.***> wrote:
Hi Fengchao, Thanks. How to get the STY table in output? I am using Timstofpro data. Best, Rajesh
Rajesh Kumar Soni , PhD Proteomics Core Manager Lasker Biomedical Research Building 3960 Broadway, 3rd Floor, Room 350D https://www.google.com/maps/search/3960+Broadway,+3rd+Floor,+Room+350D?entry=gmail&source=g (Entrance on 166th St.) Columbia University Medical Center New York, NY 10032 Email: @.*** Tel: 212-342-4177
From: Fengchao @.> Sent: Thursday, August 5, 2021 1:21 PM To: Nesvilab/FragPipe @.> Cc: Soni, Rajesh K. @.>; Author @.> Subject: [EXTERNAL] Re: [Nesvilab/FragPipe] Stopped (#419)
It's hard to tell because it depends on your variable modifications etc. You can try different values from small to large.
Best,
Fengchao
On Thu, Aug 5, 2021 at 1:19 PM rajeshksoni11 @.***> wrote:
Hi Fengcho, I am using the UniProt mouse database, how much value I should keep for spilt database? Best, Rajesh
Rajesh Kumar Soni , PhD Proteomics Core Manager Lasker Biomedical Research Building 3960 Broadway, 3rd Floor, Room 350D https://www.google.com/maps/search/3960+Broadway,+3rd+Floor,+Room+350D?entry=gmail&source=g (Entrance on 166th St.) Columbia University Medical Center New York, NY 10032 Email: @.*** Tel: 212-342-4177
From: Fengchao @.> Sent: Thursday, August 5, 2021 1:09 PM To: Nesvilab/FragPipe @.> Cc: Soni, Rajesh K. @.>; Author @.> Subject: [EXTERNAL] Re: [Nesvilab/FragPipe] Stopped (#419)
Hi Rajesh,
Non-specific search requires lots of memory. If 64 GB is not enough for your data, you can increase the value for "split database" in MSFragger tab (the default value is 1 meaning no splitting).
Best,
Fengchao
On Thu, Aug 5, 2021 at 1:05 PM rajeshksoni11 @.***> wrote:
Hi all, Thanks. I am uploading the folder, and another issue, java.lang.OutOfMemoryError, all the time if I search for a non-specific HLA search. I have 64GB ram and selected Ram= o(auto) and parallism= 10 Please suggest I fix the issues. best, Rajesh
From: Fengchao @.> Sent: Thursday, August 5, 2021 11:35 AM To: Nesvilab/FragPipe @.> Cc: Soni, Rajesh K. @.>; Author @.> Subject: [EXTERNAL] Re: [Nesvilab/FragPipe] Stopped (#419)
Thanks for your report. Can you upload your .d folders, fasta file, and fragger.params to https://www.dropbox.com/request/blovHST1fE9mOvuMM13x ?
Best,
Fengchao
On Thu, Aug 5, 2021 at 11:31 AM rajeshksoni11 @.***> wrote:
-
Describe the log_2021-08-05_11-29-12.txt <
https://github.com/Nesvilab/FragPipe/files/6939813/log_2021-08-05_11-29-12.txt
issue or question:
Upload your log file (If a log file hasn't been generated, go to the 'Run' tab in FragPipe, click 'Export Log', zip the resulting "log_[date_time].txt" file to avoid truncation, then attach the zipped file by drag & drop here.)
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Fixed. Added to v4.1.1
Describe the log_2021-08-05_11-29-12.txt issue or question:
Upload your log file (If a log file hasn't been generated, go to the 'Run' tab in FragPipe, click 'Export Log', zip the resulting "log_[date_time].txt" file to avoid truncation, then attach the zipped file by drag & drop here.)