Nesvilab / FragPipe

A cross-platform proteomics data analysis suite
http://fragpipe.nesvilab.org
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Bruker Data Format #445

Closed hmehmetkayili closed 3 years ago

hmehmetkayili commented 3 years ago

Dear Developers, We have a Bruker TIMS-TOF MS system. We tried to analyze our data (these data was obtained from QTOF experiments without ion mobility) with Fragpipe including MSfragger 3.3 and philosopher for N-glycopeptide analysis using glyco-N-LFQ workflow. When we insert our data to FragPipe as .d format ,we got an error masssage described below:

Failed in checking ...d Not a valid pathDir for Bruker data directory Failed in checking spectral files.

How we solve the problem? Should we convert the data to mzML file format as described in the tutorial?

Best, Mehmet

fcyu commented 3 years ago

We don’t support the .d without ion mobility. Please convert it to mzML with msconverter.

Best,

Fengchao

On Fri, 27 Aug 2021 at 6:55 AM, hmehmetkayili @.***> wrote:

We have a Bruker TIMS-TOF MS system. We tried to analyze our data (these data was obtained from QTOF experiments without ion mobility) with Fragpipe with MSfragger and phlisopeher for N-glycopeptide analysis using glyco-N-LFQ workflow. When we insert our data to FragPipe as .d format ,we got an error masssage described below:

Failed in checking ...d Not a valid pathDir for Bruker data directory Failed in checking spectral files.

log_2021-08-27_13-54-39.txt https://github.com/Nesvilab/FragPipe/files/7066420/log_2021-08-27_13-54-39.txt

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