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Nesvilab / FragPipe

A cross-platform Graphical User Interface (GUI) for running MSFragger and Philosopher - powered pipeline for comprehensive analysis of shotgun proteomics data
http://fragpipe.nesvilab.org
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open search: wrong precursor mass tolerance #58

Closed ThomasGossenreiter closed 6 years ago

ThomasGossenreiter commented 6 years ago

Describe the problem

I want to do an open search from 0 to 1000 Da precursor tolerance. I set precursor_mass_lower = 0 and precursor_mass_upper = 1000. However the modification list contains range from -500 to 500, like the default settings. Am I doing anything wrong? How can I solve this issue? The aim is to use MSfragger for the identification of protein-RNA cross-links. Thank you in advance!

System info

Input data files

mzML, used the Henning Urlaub benchmark files from ProteomeXchange: XL_human_RBPs_UV.mzML XL_human_RBPs_control.mzML

Sequence database

human, used philosopher to generate decoys

Attach fragger.params file

fragger.params.txt

andytyk commented 6 years ago

Hi Thomas,

Can you attach the output from the GUI? Also, check to make sure you have the latest version of MSFragger - you should be able to update it from the GUI or manually via our upgrader site (http://msfragger.arsci.com/upgrader/).

Andy

ThomasGossenreiter commented 6 years ago

Hi Andy,

thanks for your reply.

I am using the latest version: MSFragger-20180316.jar

I attached the output files, except the pep.xml, since its too big for e-mail.

Thank you very much for your help!

Thomas

Am 18.04.2018 16:28, schrieb Andy Kong:

Hi Thomas,

Can you attach the output from the GUI? Also, check to make sure you have the latest version of MSFragger - you should be able to update it from the GUI or manually via our upgrader site (http://msfragger.arsci.com/upgrader/).

Andy

-- You are receiving this because you authored the thread. Reply to this email directly, view it on GitHub [1], or mute the thread [2].

*

Links:

[1] https://github.com/chhh/MSFragger-GUI/issues/58#issuecomment-382406438 [2] https://github.com/notifications/unsubscribe-auth/AkrTJEW8Ed10PBZj2lGzQCkXFwjRjAnkks5tp02PgaJpZM4TZhJr

-- Thomas Gossenreiter, MSc Mass Spectrometry Facility Max F. Perutz Laboratories Vienna Biocenter (VBC) Dr. Bohr Gasse 3 (room 5.10) 1030 Vienna, Austria num_threads = 2 # 0=poll CPU to set num threads; else specify num threads directly (max 64) precursor_mass_tolerance = 500.00 precursor_mass_lower = -100 # Overrides the lower bound of the window set by precursor_mass_tolerance precursor_mass_upper = 1000 # Overrides the upper bound of the window set by precursor_mass_tolerance precursor_mass_units = 0 # 0=Daltons, 1=ppm precursor_true_tolerance = 10 precursor_true_units = 1 # 0=Daltons, 1=ppm fragment_mass_tolerance = 20 fragment_mass_units = 1 # 0=Daltons, 1=ppm isotope_error = 0 # 0=off, -1/0/1/2/3 (standard C13 error) mass_offsets = 0 # allow for additional precursor mass window shifts. Multiplexed with isotope_error. mass_offsets = 0/79.966 can be used as a restricted ‘open’ search that looks for unmodified and phosphorylated peptides (on any residue) search_enzyme_name = Trypsin search_enzyme_cutafter = KR search_enzyme_butnotafter = P num_enzyme_termini = 2 # 2 for enzymatic, 1 for semi-enzymatic, 0 for nonspecific digestion allowed_missed_cleavage = 1 # maximum value is 5 clip_nTerm_M = 1 variable_mod_01 = 15.99490 M variable_mod_02 = 42.01060 [^

variable_mod_03 = 79.96633 STY

variable_mod_04 = -17.02650 nQnC

variable_mod_05 = -18.01060 nE

allow_multiple_variable_mods_on_residue = 1 # static mods are not considered max_variable_mods_per_mod = 3 # maximum of 5 max_variable_mods_combinations = 5000 # maximum of 65534, limits number of modified peptides generated from sequence output_file_extension = pepXML output_format = pepXML output_report_topN = 1 output_max_expect = 50.0 precursor_charge = 0 0 # precursor charge range to analyze; does not override any existing charge; 0 as 1st entry ignores parameter override_charge = 0 # 0=no, 1=yes to override existing precursor charge states with precursor_charge parameter digest_min_length = 7 digest_max_length = 50 digest_mass_range = 500.0 5000.0 # MH+ peptide mass range to analyze max_fragment_charge = 2 # set maximum fragment charge state to analyze (allowed max 5) track_zero_topN = 0 # in addition to topN results, keep track of top results in zero bin zero_bin_accept_expect = 0 # boost top zero bin entry to top if it has expect under 0.01 - set to 0 to disable zero_bin_mult_expect = 1 # disabled if above passes - multiply expect of zero bin for ordering purposes (does not affect reported expect) add_topN_complementary = 0 minimum_peaks = 15 # required minimum number of peaks in spectrum to search (default 10) use_topN_peaks = 100 min_fragments_modelling = 3 min_matched_fragments = 6 minimum_ratio = 0.01 # filter peaks below this fraction of strongest peak clear_mz_range = 0.0 0.0 # for iTRAQ/TMT type data; will clear out all peaks in the specified m/z range add_Cterm_peptide = 0.000000 add_Nterm_peptide = 0.000000 add_Cterm_protein = 0.000000 add_Nterm_protein = 0.000000 add_G_glycine = 0.000000 add_A_alanine = 0.000000 add_S_serine = 0.000000 add_P_proline = 0.000000 add_V_valine = 0.000000 add_T_threonine = 0.000000 add_C_cysteine = 57.021464 add_L_leucine = 0.000000 add_I_isoleucine = 0.000000 add_N_asparagine = 0.000000 add_D_aspartic_acid = 0.000000 add_Q_glutamine = 0.000000 add_K_lysine = 0.000000 add_E_glutamic_acid = 0.000000 add_M_methionine = 0.000000 add_H_histidine = 0.000000 add_F_phenylalanine = 0.000000 add_R_arginine = 0.000000 add_Y_tyrosine = 0.000000 add_W_tryptophan = 0.000000 add_B_user_amino_acid = 0.000000 add_J_user_amino_acid = 0.000000 add_O_user_amino_acid = 0.000000 add_U_user_amino_acid = 0.000000 add_X_user_amino_acid = 0.000000 add_Z_user_amino_acid = 0.000000 database_name = C:\MSFragger\2018-04-16-td-UP000005640.fas

Peptide Sequence Modified Sequence M/Z Charge Experimental Mass Probability Expectation Spectral Count Unmodified Observations Modified Observations Intensity Assigned Modifications Observed Modifications Intensity Mapped Proteins Protein IDs AAAAVVEFQR n[43]AAAAVVEFQR 552.2958 2 1102.5771 1.0000 0.0000 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|O00231|PSD11_HUMAN AAAGGAEGRR n[43]AAAGGAEGRR 479.2467 2 956.4788 0.9996 0.0011 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|Q86UK7|ZN598_HUMAN AAAMANNLQK AAAM[147]ANNLQK 524.2662 2 1046.5178 0.9378 0.6895 1 1 0 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|Q14498|RBM39_HUMAN AAATKKPAPEK 371.2204 3 1110.6395 0.9961 0.0001 1 0 1 0.0000 44.0085:Delta:H(4)C(2)O(-1)S(1) (S-Ethylcystine from Serine), 44.0085:Ser->Met (Ser->Met substitution), 44.0173:Acetyl:13C(2) (heavy acetylation) 0.0000 1 sp|P36578|RL4_HUMAN AADHLKPFLDDSTLR 566.9652 3 1697.8737 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|O43395|PRPF3_HUMAN AADLLVNPLDPR 647.3616 2 1292.7086 0.9948 0.0388 1 1 0 0.0000 Unknown 0.0000 1 sp|P78362|SRPK2_HUMAN AADPPAENSSAPEAEQGGAE 633.2731 3 1896.7974 1.0000 0.0000 2 1 1 0.0000 Unknown 0.0000 1 sp|P67809|YBOX1_HUMAN AADPPAENSSAPEAEQGGAE 949.4060 2 1896.7974 1.0000 0.0000 3 1 2 0.0000 Unknown 0.0000 1 sp|P67809|YBOX1_HUMAN AADVSVTHRPPLSPK n[43]AADVSVTHRPPLSPK 539.6299 3 1615.8680 1.0000 0.0000 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|Q8IY67|RAVR1_HUMAN AAEEQLSAR 487.7487 2 973.4828 0.9998 0.0007 1 1 0 0.0000 Unknown 0.0000 1 sp|Q96CS2|HAUS1_HUMAN AAGFERPSPVQLK 467.2612 3 1398.7618 0.9679 0.0953 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|Q9UHI6|DDX20_HUMAN AAGKGDVPTK 472.2640 2 942.5134 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P62424|RL7A_HUMAN AAGVVLEMIR 529.8049 2 1057.5953 0.9989 0.0031 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9Y230|RUVB2_HUMAN AAHSEGNTTAGLDMR AAHSEGNTTAGLDM[147]R 516.2353 3 1545.6841 1.0000 0.0000 2 2 0 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|P78371|TCPB_HUMAN AAHSEGNTTAGLDMR AAHSEGNTTAGLDM[147]R 773.8493 2 1545.6841 0.9984 0.0114 2 1 1 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|P78371|TCPB_HUMAN AAILPTSIFLTNK 694.9110 2 1387.8075 1.0000 0.0004 1 1 0 0.0000 Unknown 0.0000 1 sp|O14744|ANM5_HUMAN AAILPTSIFLTNKK 506.3081 3 1515.9025 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|O14744|ANM5_HUMAN AALEAVGGTVVLE 614.8428 2 1227.6710 1.0000 0.0000 2 1 1 0.0000 17.0345:Methyl:2H(3) (deuterated methyl ester), 17.0265:Ammonium (replacement of proton with ammonium ion) 0.0000 1 sp|P52815|RM12_HUMAN AALEEVYPDLTPEETRR 663.6689 3 1987.9850 0.9986 0.0040 2 2 0 0.0000 Unknown 0.0000 1 sp|Q9H0D6|XRN2_HUMAN AALKADAQD 451.7325 2 901.4504 0.9971 0.0042 1 1 0 0.0000 Unknown 0.0000 1 sp|P82663|RT25_HUMAN AALQELLSK 486.7898 2 971.5651 0.9987 0.0071 2 2 0 0.0000 Unknown 0.0000 1 sp|P62851|RS25_HUMAN AALRPLVKPK 364.9132 3 1091.7178 0.9995 0.0019 2 1 1 0.0000 Unknown 0.0000 1 sp|P62910|RL32_HUMAN AALTRDPQFQK n[43]AALTRDPQFQK 658.8515 2 1315.6884 1.0000 0.0000 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|P06744|G6PI_HUMAN AALVLEDGSVLR n[43]AALVLEDGSVLR 642.8615 2 1283.7085 0.9981 0.0143 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|P27708|PYR1_HUMAN AAMAVGGAGGSR n[43]AAMAVGGAGGSR 523.7561 2 1045.4976 1.0000 0.0001 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|O14744|ANM5_HUMAN AAMAVGGAGGSR n[43]AAM[147]AVGGAGGSR 531.7535 2 1061.4924 0.9987 0.0066 1 1 0 0.0000 42.0106:Acetyl (Acetylation), 15.9949:Oxidation (Oxidation or Hydroxylation) -0.0364:Lys->Gln (Lys->Gln substitution) 0.0000 1 sp|O14744|ANM5_HUMAN AANPVDFLSK 531.2849 2 1060.5552 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q8IY81|SPB1_HUMAN AAPDILIATPGR 597.8456 2 1193.6766 0.9588 0.4874 1 1 0 0.0000 Unknown 0.0000 1 sp|Q96GQ7|DDX27_HUMAN AAPGAEFAPNKR 614.8252 2 1227.6359 0.9994 0.0014 2 1 1 0.0000 Unknown 0.0000 1 sp|Q15233|NONO_HUMAN AAPGAEFAPNKR 410.2192 3 1227.6359 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q15233|NONO_HUMAN AAPPPPPPPPPLESSPR 568.6419 3 1702.9039 0.9966 0.0127 1 1 0 0.0000 Unknown 0.0000 1 sp|Q5T4S7|UBR4_HUMAN AAPVDLELKK n[43]AAPVDLELKK 563.3293 2 1124.6440 1.0000 0.0001 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|O60925|PFD1_HUMAN AAQDRDQIYR 412.5424 3 1234.6053 0.9999 0.0005 1 1 0 0.0000 Unknown 0.0000 1 sp|P62995|TRA2B_HUMAN AAQDRDQIYR 618.3099 2 1234.6053 0.9514 0.1678 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P62995|TRA2B_HUMAN AAQLAIRVTNPNAR 498.9548 3 1493.8425 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P62910|RL32_HUMAN AASDIAMTELPPTHPIR 607.3172 3 1818.9297 1.0000 0.0000 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P62258|1433E_HUMAN AASDIAMTELPPTHPIR AASDIAM[147]TELPPTHPIR 612.6488 3 1834.9246 1.0000 0.0000 4 2 2 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P62258|1433E_HUMAN AATPQVAAELEK 614.3325 2 1226.6505 1.0000 0.0000 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|O95900|TRUB2_HUMAN AAVAGKKPVVGK 375.5765 3 1123.7076 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P36578|RL4_HUMAN AAVAGKKPVVGK 562.8611 2 1123.7076 1.0000 0.0000 2 1 1 0.0000 Unknown 0.0000 1 sp|P36578|RL4_HUMAN AAVELEEPEDAR 664.8200 2 1327.6255 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|O94906|PRP6_HUMAN AAVEWFDGKDFQGSK 562.2703 3 1683.7892 1.0000 0.0000 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|Q01844|EWS_HUMAN AAVLLEQER 514.7904 2 1027.5662 0.9995 0.0012 1 1 0 0.0000 Unknown 0.0000 1 sp|Q13435|SF3B2_HUMAN AAVSAFPTDSLER 682.3462 2 1362.6779 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|O14654|IRS4_HUMAN AAVVENVVK n[43]AAVVENVVK 485.7821 2 969.5496 0.9934 0.0530 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|Q92785|REQU_HUMAN ADAEVIILPK n[43]ADAEVIILPK 555.8238 2 1109.6331 1.0000 0.0002 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|O60832|DKC1_HUMAN ADAPEEEDHVLVLR 531.6020 3 1591.7841 1.0000 0.0000 1 0 1 0.0000 -71.0371:Gln->Gly (Gln->Gly substitution) 0.0000 1 sp|P07237|PDIA1_HUMAN ADAPEEEDHVLVLR 796.8993 2 1591.7841 1.0000 0.0000 1 0 1 0.0000 Unknown 0.0000 1 sp|P07237|PDIA1_HUMAN ADDADEFGYSR 623.2545 2 1244.4944 0.9991 0.0012 1 0 1 0.0000 Unknown 0.0000 1 sp|Q7KZF4|SND1_HUMAN ADEGISFR 447.7194 2 893.4243 0.9987 0.0052 1 1 0 0.0000 Unknown 0.0000 1 sp|Q06830|PRDX1_HUMAN ADEVLEILK 515.2949 2 1028.5753 0.9974 0.0190 1 1 0 0.0000 Unknown 0.0000 1 sp|O75643|U520_HUMAN ADGIVSK 345.1950 2 688.3755 0.9133 0.2001 1 0 1 0.0000 128.0950:Lys (Addition of lysine due to transpeptidation) 0.0000 1 sp|P63220|RS21_HUMAN ADGTATAPPPR 527.2697 2 1052.5249 0.9977 0.0170 1 1 0 0.0000 Unknown 0.0000 1 sp|Q7Z6Z7|HUWE1_HUMAN ADGYEPPVQESV 645.7959 2 1289.5773 1.0000 0.0000 4 3 1 0.0000 17.0345:Methyl:2H(3) (deuterated methyl ester), 17.0265:Ammonium (replacement of proton with ammonium ion) 0.0000 1 sp|P61247|RS3A_HUMAN ADGYNQPDSK 547.7410 2 1093.4675 1.0000 0.0000 3 3 0 0.0000 Unknown 0.0000 1 sp|O43390|HNRPR_HUMAN ADGYVLEGK 476.2427 2 950.4709 1.0000 0.0001 3 1 2 0.0000 Unknown 0.0000 1 sp|P62241|RS8_HUMAN ADHSFSDGVPSDSVEAAK n[43]ADHSFSDGVPSDSVEAAK 930.9159 2 1859.8173 1.0000 0.0001 2 0 2 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|Q99733|NP1L4_HUMAN ADKEAAFDDAVEER n[43]ADKEAAFDDAVEER 804.3627 2 1606.7109 1.0000 0.0000 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|Q09028|RBBP4_HUMAN ADLEEQLSDEEKVR n[43]ADLEEQLSDEEKVR 851.9101 2 1701.8057 0.9999 0.0004 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|P47755|CAZA2_HUMAN ADLINNLGTIAK 621.8562 2 1241.6979 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P07900|HS90A_HUMAN ADQLTEEQIAEFK n[43]ADQLTEEQIAEFK 782.3804 2 1562.7463 1.0000 0.0000 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|P0DP23|CALM1_HUMAN ADSGPTQPPLSLSPAPETK 946.9835 2 1891.9525 0.9987 0.0054 2 1 1 0.0000 Unknown 0.0000 1 sp|O15027|SC16A_HUMAN ADSWVEKEEPAPSN 779.8546 2 1557.6946 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q8N163|CCAR2_HUMAN ADTQTYQPYNKDWIK 624.3039 3 1869.8899 1.0000 0.0000 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P84090|ERH_HUMAN ADTQTYQPYNKDWIK 935.9522 2 1869.8899 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P84090|ERH_HUMAN ADVQSIIGLQR 600.3407 2 1198.6669 0.9925 0.0651 1 1 0 0.0000 Unknown 0.0000 1 sp|Q13263|TIF1B_HUMAN AEAIVYK 397.2264 2 792.4382 0.9959 0.0302 1 1 0 0.0000 Unknown 0.0000 1 sp|P82933|RT09_HUMAN AEAPGQPLKPPEGQEGFSQR 708.3553 3 2122.0442 0.9999 0.0001 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9UEG4|ZN629_HUMAN AEDEVQRER 377.8511 3 1130.5315 0.9823 0.0201 1 0 1 0.0000 128.0950:Lys (Addition of lysine due to transpeptidation) 0.0000 1 sp|P0DMV8|HS71A_HUMAN AEDKEWMPVTK AEDKEWM[147]PVTK 450.5517 3 1348.6332 1.0000 0.0000 2 2 0 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|P15880|RS2_HUMAN AEEEIMKK AEEEIM[147]KK 497.2497 2 992.4848 0.8930 1.1690 1 1 0 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|O00425|IF2B3_HUMAN AEEEIMKK 489.2522 2 976.4899 1.0000 0.0004 1 1 0 0.0000 Unknown 0.0000 1 sp|O00425|IF2B3_HUMAN AEEELSIDKVLESEQDK 491.2442 4 1960.9476 0.8552 3.6000 1 0 1 0.0000 Unknown 0.0000 1 sp|O43432|IF4G3_HUMAN AEEPSDLIGPEAPK 726.8644 2 1451.7142 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q8N5F7|NKAP_HUMAN AEFTSPPSLFK 612.3189 2 1222.6233 0.9987 0.0051 1 1 0 0.0000 Unknown 0.0000 1 sp|Q6UN15|FIP1_HUMAN AEGGGGGGRPGAPAAGDGKTEQK 675.6630 3 2023.9672 1.0000 0.0000 2 0 2 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|Q00839|HNRPU_HUMAN AEGGGGGGRPGAPAAGDGKTEQK 506.9991 4 2023.9672 1.0000 0.0000 1 0 1 0.0000 128.0950:Lys (Addition of lysine due to transpeptidation) 0.0000 1 sp|Q00839|HNRPU_HUMAN AEGIREPK 450.2509 2 898.4872 0.9992 0.0010 1 1 0 0.0000 Unknown 0.0000 1 sp|P46087|NOP2_HUMAN AEGPGRGDEPSPS 628.2810 2 1254.5475 0.9783 0.1075 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9NVI7|ATD3A_HUMAN AEGYAEGDLTLYHR 532.2547 3 1593.7423 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q8IY81|SPB1_HUMAN AEKTLGDFAAEYAK 379.1938 4 1512.7460 0.9998 2.2700 1 0 1 0.0000 Unknown 0.0000 1 sp|P09874|PARP1_HUMAN AELDNELMEGK 416.8640 3 1247.5703 0.8355 0.0916 1 0 1 0.0000 Unknown 0.0000 1 sp|Q13523|PRP4B_HUMAN AELGEADEAELQR n[43]AELGEADEAELQR 736.8468 2 1471.6790 1.0000 0.0000 1 0 1 0.0000 42.0106:Acetyl (Acetylation) 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|Q9Y5J9|TIM8B_HUMAN AELIQKK n[43]AELIQKK 436.2660 2 870.5175 0.9965 0.0253 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|O15212|PFD6_HUMAN AELNEFLTR 546.7878 2 1091.5610 1.0000 0.0002 1 1 0 0.0000 Unknown 0.0000 1 sp|P23396|RS3_HUMAN AEPVEVVAPR 533.7982 2 1065.5818 0.9993 0.0016 1 1 0 0.0000 Unknown 0.0000 1 sp|P09874|PARP1_HUMAN AEQEIMKK 488.7602 2 975.5059 0.9879 0.0507 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|Q9NZI8|IF2B1_HUMAN AERDSALETLQGQLEEK 639.6568 3 1915.9487 0.8543 0.7626 1 1 0 0.0000 Unknown 0.0000 1 sp|Q14980|NUMA1_HUMAN AEVEGKDLPEHAVLK 409.4741 4 1633.8673 1.0000 0.0000 1 0 1 0.0000 128.0950:Lys (Addition of lysine due to transpeptidation) 0.0000 1 sp|Q00839|HNRPU_HUMAN AEVEGKDLPEHAVLK 545.6297 3 1633.8673 0.9984 0.0010 1 0 1 0.0000 Unknown 0.0000 1 sp|Q00839|HNRPU_HUMAN AEVEQKK n[43]AEVEQKK 437.2375 2 872.4604 0.9988 0.0040 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|P62841|RS15_HUMAN AEVEQVELPDGK 657.3328 2 1312.6510 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9NW13|RBM28_HUMAN AEYDLTTR n[43]AEYDLTTR 505.7431 2 1009.4716 0.9960 0.0288 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|P60228|EIF3E_HUMAN AFADSSYLLR 571.7956 2 1141.5767 1.0000 0.0004 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9H5H4|ZN768_HUMAN AFDLFNPNFK 606.8059 2 1211.5973 0.9988 0.0045 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9Y3D9|RT23_HUMAN AFGDSSYLLR 564.7878 2 1127.5610 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9H5H4|ZN768_HUMAN AFGSGYR 379.1850 2 756.3554 0.8888 1.2190 1 1 0 0.0000 Unknown 0.0000 1 sp|P23588|IF4B_HUMAN AFGYYGPLR 522.2691 2 1042.5236 0.9989 0.0032 2 1 1 0.0000 Unknown 0.0000 1 sp|P84103|SRSF3_HUMAN AFHNEAQVNPER 706.3392 2 1410.6639 1.0000 0.0000 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P17987|TCPA_HUMAN AFHNEAQVNPER 471.2286 3 1410.6639 1.0000 0.0000 2 1 1 0.0000 Unknown 0.0000 1 sp|P17987|TCPA_HUMAN AFHNEAQVNPERK 513.9269 3 1538.7589 1.0000 0.0000 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P17987|TCPA_HUMAN AFHNEAQVNPERK 770.3867 2 1538.7589 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P17987|TCPA_HUMAN AFITNIPFDVK 632.8504 2 1263.6862 0.9962 0.0149 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P52272|HNRPM_HUMAN AFKDTGK n[43]AFKDTGK 404.7136 2 807.4127 0.8941 0.5457 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|P60866|RS20_HUMAN AFLIEEQK 489.2687 2 976.5229 0.8960 0.6449 1 0 1 0.0000 Unknown 0.0000 1 sp|P49207|RL34_HUMAN AFLIEEQKIVVK 472.9535 3 1415.8386 1.0000 0.0000 2 2 0 0.0000 Unknown 0.0000 1 sp|P49207|RL34_HUMAN AFMGPLKK AFM[147]GPLKK 454.2571 2 906.4996 0.9988 0.0035 1 1 0 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|P39023|RL3_HUMAN AFNDPFIQK 540.2796 2 1078.5447 0.9979 0.0155 2 1 1 0.0000 Unknown 0.0000 1 sp|Q92598|HS105_HUMAN AFSDPFVEAEK 620.2982 2 1238.5818 1.0000 0.0003 1 1 0 0.0000 Unknown 0.0000 1 sp|P34932|HSP74_HUMAN AFSQFGK 392.7030 2 783.3915 0.9903 0.1228 1 1 0 0.0000 Unknown 0.0000 1 sp|O60506|HNRPQ_HUMAN AFSQFGKLER 394.8804 3 1181.6193 1.0000 0.0000 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|O60506|HNRPQ_HUMAN AFSQNTGLILHQR 495.6037 3 1483.7894 0.9996 0.0001 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|Q15776|ZKSC8_HUMAN AFSQSSDLIK 548.2876 2 1094.5607 0.9965 0.0254 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9H5H4|ZN768_HUMAN AFSYYGPLR 537.2743 2 1072.5341 0.9991 0.0022 1 1 0 0.0000 Unknown 0.0000 1 sp|Q16629|SRSF7_HUMAN AGAGPGGPPQKPAPSSQR 553.9568 3 1658.8486 0.9998 0.0002 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9Y5J1|UTP18_HUMAN AGAHLQGGAKR 533.2992 2 1064.5839 0.9930 0.0571 1 1 0 0.0000 Unknown 0.0000 1 sp|P04406|G3P_HUMAN AGAKPDQIGIITPYEGQR 638.6741 3 1913.0006 0.9955 0.0077 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|Q92900|RENT1_HUMAN AGDRQPEWLEEQQGR 600.2871 3 1797.8394 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q14980|NUMA1_HUMAN AGEAPTENPAPPTQQSSAE 941.4266 2 1880.8386 1.0000 0.0000 2 0 2 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P16989|YBOX3_HUMAN AGEKVEKPDTK n[43]AGEKVEKPDTK 622.3300 2 1242.6455 1.0000 0.0000 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|Q02878|RL6_HUMAN AGEKVEKPDTK 401.2189 3 1200.6349 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q02878|RL6_HUMAN AGEKVEKPDTK 601.3247 2 1200.6349 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q02878|RL6_HUMAN AGELTEDEVER 624.2910 2 1246.5675 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P62269|RS18_HUMAN AGFAGDDAPR 326.1542 3 975.4409 1.0000 0.0000 1 0 1 0.0000 Unknown 0.0000 1 sp|A5A3E0|POTEF_HUMAN AGFAGDDAPR 488.7277 2 975.4409 0.9990 0.0024 1 1 0 0.0000 Unknown 0.0000 1 sp|A5A3E0|POTEF_HUMAN AGFRPVEAGGQHGGR 499.2553 3 1494.7440 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|O15234|CASC3_HUMAN AGGEAGVTLGQPHLSR n[43]AGGEAGVTLGQPHLSR 796.4130 2 1590.8114 1.0000 0.0000 1 0 1 0.0000 42.0106:Acetyl (Acetylation) 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P41091|IF2G_HUMAN AGGIETIANEYSDR 748.3547 2 1494.6948 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P34932|HSP74_HUMAN AGGSAALSPSK 473.2536 2 944.4927 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q92522|H1X_HUMAN AGIEGPLLASDVGR 677.8698 2 1353.7250 0.9990 0.0027 1 1 0 0.0000 Unknown 0.0000 1 sp|P18583|SON_HUMAN AGKFPSLLTHNENMVAK 619.6611 3 1855.9614 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P62906|RL10A_HUMAN AGKFPSLLTHNENMVAK 464.9976 4 1855.9614 1.0000 0.0000 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P62906|RL10A_HUMAN AGKFPSLLTHNENMVAK AGKFPSLLTHNENM[147]VAK 624.9927 3 1871.9563 1.0000 0.0000 3 1 2 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) -1.0078:Dehydro (Half of a disulfide bridge) 0.0000 1 sp|P62906|RL10A_HUMAN AGLKEAPEGWQTPK 756.3963 2 1510.7780 1.0000 0.0002 2 1 1 0.0000 Unknown 0.0000 1 sp|Q9NWT8|AKIP_HUMAN AGLLPNHRPR 377.5562 3 1129.6467 0.9973 0.0086 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9NVS2|RT18A_HUMAN AGLLQPPVR 475.7927 2 949.5709 0.9842 0.0832 2 2 0 0.0000 Unknown 0.0000 1 sp|Q8IX12|CCAR1_HUMAN AGLQFPVGR 472.7692 2 943.5239 1.0000 0.0002 2 1 1 0.0000 Unknown 0.0000 1 sp|P04908|H2A1B_HUMAN AGLSQSLFER 554.2932 2 1106.5719 0.9992 0.0017 1 1 0 0.0000 Unknown 0.0000 1 sp|Q92900|RENT1_HUMAN AGMTHIVR AGM[147]THIVR 450.7396 2 899.4647 1.0000 0.0000 1 1 0 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|P39023|RL3_HUMAN AGNFYVPAEPK 596.8035 2 1191.5924 0.9987 0.0051 1 1 0 0.0000 Unknown 0.0000 1 sp|P18124|RL7_HUMAN AGNGQWASVIR 579.8043 2 1157.5941 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q15393|SF3B3_HUMAN AGNLGGGVVTIER 621.8436 2 1241.6726 1.0000 0.0000 2 1 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P35268|RL22_HUMAN AGPEYGQGMNPISR 738.8486 2 1475.6827 0.9999 0.0005 1 0 1 0.0000 Unknown 0.0000 1 sp|Q9NUL3|STAU2_HUMAN AGPIWDLR 464.2559 2 926.4973 0.9955 0.0332 2 2 0 0.0000 Unknown 0.0000 1 sp|O43390|HNRPR_HUMAN AGRGFSLEELR 412.2228 3 1233.6465 0.9974 0.0120 1 1 0 0.0000 Unknown 0.0000 1 sp|P26373|RL13_HUMAN AGTGVDNVDLEAATR 744.8680 2 1487.7214 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|O43175|SERA_HUMAN AGTVVLDDVELR n[43]AGTVVLDDVELR 664.8564 2 1327.6982 0.9996 0.0010 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|P25205|MCM3_HUMAN AGVAPLQVK 441.7740 2 881.5334 1.0000 0.0001 2 1 1 0.0000 Unknown 0.0000 1 sp|P21333|FLNA_HUMAN AGVNTVTTLVENK 673.3697 2 1344.7249 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P62424|RL7A_HUMAN AGVNTVTTLVENKK 491.9472 3 1472.8198 1.0000 0.0000 1 1 0 0.0000 0.9840:Deamidated (Deamidation), 0.9840:Asn->Asp (Asn->Asp substitution), 0.9840:Gln->Glu (Gln->Glu substitution) 0.0000 1 sp|P62424|RL7A_HUMAN AGVNTVTTLVENKK 737.4172 2 1472.8198 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P62424|RL7A_HUMAN AHFTIWDK 509.2612 2 1016.5079 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9NYK5|RM39_HUMAN AHGNSGMVR AHGNSGM[147]VR 472.7220 2 943.4294 0.8573 1.6510 1 1 0 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|P18077|RL35A_HUMAN AHLGTALK 405.7452 2 809.4759 1.0000 0.0002 1 1 0 0.0000 Unknown 0.0000 1 sp|P62266|RS23_HUMAN AHSIQIMK AHSIQIM[147]K 472.2551 2 942.4957 0.9999 0.0006 1 1 0 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|Q02543|RL18A_HUMAN AHTPQVHK 459.2512 2 916.4879 0.9964 0.0259 1 1 0 0.0000 Unknown 0.0000 1 sp|Q8TCC3|RM30_HUMAN AHWTPFEGQK 600.7934 2 1199.5723 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P27708|PYR1_HUMAN AHWTPFEGQK 400.8647 3 1199.5723 0.9999 0.0004 1 1 0 0.0000 Unknown 0.0000 1 sp|P27708|PYR1_HUMAN AIADTGANVVVTGGK 686.8752 2 1371.7358 1.0000 0.0000 3 2 1 0.0000 Unknown 0.0000 1 sp|P50990|TCPQ_HUMAN AIADWYNEK 555.2667 2 1108.5188 0.9994 0.0014 1 1 0 0.0000 Unknown 0.0000 1 sp|P10155|RO60_HUMAN AIADYLR 411.2294 2 820.4443 0.9985 0.0103 1 1 0 0.0000 Unknown 0.0000 1 sp|P43034|LIS1_HUMAN AIEDEGGNPDEIEITSEGNKK 749.0204 3 2244.0393 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q15424|SAFB1_HUMAN AIEFLNNPPEEAPR 798.9043 2 1595.7941 1.0000 0.0000 2 2 0 0.0000 Unknown 0.0000 1 sp|Q4G0J3|LARP7_HUMAN AIETFSGK 426.7267 2 851.4389 0.9917 0.0817 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9NZI8|IF2B1_HUMAN AIETFSGKVELQGK 753.9118 2 1505.8090 1.0000 0.0000 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|Q9NZI8|IF2B1_HUMAN AIETLSGKVELHGK 494.6156 3 1480.8250 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9Y6M1|IF2B2_HUMAN AIETTDIISR 559.8062 2 1117.5978 0.9991 0.0021 2 1 1 0.0000 Unknown 0.0000 1 sp|Q96HS1|PGAM5_HUMAN AIFAGYK 385.2158 2 768.4170 0.9948 0.0388 1 1 0 0.0000 Unknown 0.0000 1 sp|P18077|RL35A_HUMAN AIFAGYKR 463.2663 2 924.5181 0.9970 0.0217 2 1 1 0.0000 Unknown 0.0000 1 sp|P18077|RL35A_HUMAN AIFQQPPVGVR 606.3483 2 1210.6821 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q7L2E3|DHX30_HUMAN AIGDGEDEMLFTR AIGDGEDEM[147]LFTR 735.3324 2 1468.6503 0.9917 0.0813 2 2 0 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|O14654|IRS4_HUMAN AIGNIELGIR 528.3140 2 1054.6134 0.9995 0.0011 1 1 0 0.0000 Unknown 0.0000 1 sp|P46821|MAP1B_HUMAN AIGSASEGAQSSLQEVYHK 654.6570 3 1960.9491 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P28066|PSA5_HUMAN AIKELEEWYAR 469.9137 3 1406.7194 0.9998 0.0001 1 1 0 0.0000 Unknown 0.0000 1 sp|P09496|CLCA_HUMAN AIKVEQATKPSFESGR 437.7388 4 1746.9263 1.0000 0.0000 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P38159|RBMX_HUMAN AIKVEQATKPSFESGR 583.3160 3 1746.9263 1.0000 0.0000 1 0 1 0.0000 43.0058:Carbamyl (Carbamylation), 43.0170:Xle->Arg (Leu/Ile->Arg substitution), 43.0058:Ala->Asn (Ala->Asn substitution) 0.0000 1 sp|P38159|RBMX_HUMAN AILVDLEPGTMDSVR AILVDLEPGTM[147]DSVR 816.4190 2 1630.8234 1.0000 0.0000 5 1 4 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|P07437|TBB5_HUMAN AILVDLEPGTMDSVR 808.4215 2 1614.8285 0.9933 0.0540 1 1 0 0.0000 Unknown 0.0000 1 sp|P07437|TBB5_HUMAN AINQGGLTSVAVR 643.3647 2 1284.7148 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P60900|PSA6_HUMAN AINQQTGAFVEISR 767.4046 2 1532.7946 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q92945|FUBP2_HUMAN AINTLNGLR 486.2853 2 970.5560 0.9971 0.0087 1 0 1 0.0000 43.0058:Ala->Asn (Ala->Asn substitution), 43.0058:Carbamyl (Carbamylation) 0.0000 1 sp|P26378|ELAV4_HUMAN AIPQLQGYLR 579.8351 2 1157.6556 0.9957 0.0312 1 1 0 0.0000 Unknown 0.0000 1 sp|Q02878|RL6_HUMAN AIQDPAFSDGIR 645.3278 2 1288.6411 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q7Z6Z7|HUWE1_HUMAN AIRLELQGPR 384.8997 3 1151.6774 0.9996 0.0013 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P19338|NUCL_HUMAN AITGFDDPFSGK 627.8036 2 1253.5927 0.9962 0.0275 1 1 0 0.0000 Unknown 0.0000 1 sp|P15924|DESP_HUMAN AITHLNNNFMFGQK 545.6084 3 1633.8033 1.0000 0.0000 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P14866|HNRPL_HUMAN AITHLNNNFMFGQK AITHLNNNFM[147]FGQK 550.9400 3 1649.7982 1.0000 0.0000 4 2 2 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|P14866|HNRPL_HUMAN AITHLNNNFMFGQK 817.9089 2 1633.8033 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P14866|HNRPL_HUMAN AIVAIENPADVSVISSR 870.9780 2 1739.9414 1.0000 0.0001 1 1 0 0.0000 Unknown 0.0000 1 sp|P08865|RSSA_HUMAN AKAPIQDIWYHEDR 581.2934 3 1740.8584 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9Y3D9|RT23_HUMAN AKAPIQDIWYHEDR 436.2219 4 1740.8584 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9Y3D9|RT23_HUMAN AKDANNGNLQLR 657.3496 2 1312.6847 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P05455|LA_HUMAN AKEAAEQDVEK 406.5384 3 1216.5934 0.9984 0.0060 1 1 0 0.0000 Unknown 0.0000 1 sp|P26373|RL13_HUMAN AKEAAEQDVEK 609.3040 2 1216.5934 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P26373|RL13_HUMAN AKEFTNVYIK 404.8922 3 1211.6549 0.9954 0.0251 1 1 0 0.0000 Unknown 0.0000 1 sp|P11940|PABP1_HUMAN AKEFTNVYIK 606.8347 2 1211.6549 1.0000 0.0000 4 1 3 0.0000 43.0058:Ala->Asn (Ala->Asn substitution), 43.0058:Carbamyl (Carbamylation) 0.0000 1 sp|P11940|PABP1_HUMAN AKELLGQGLLLR 655.9113 2 1309.8081 1.0000 0.0001 2 1 1 0.0000 Unknown 0.0000 1 sp|B5ME19|EIFCL_HUMAN AKELLGQGLLLR 437.6100 3 1309.8081 0.9999 0.0004 1 1 0 0.0000 Unknown 0.0000 1 sp|B5ME19|EIFCL_HUMAN AKEQEAEPEEQEEDSSSDPR 763.9911 3 2288.9514 0.9997 0.0001 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P55081|MFAP1_HUMAN AKFEELNMDLFR AKFEELNM[147]DLFR 510.2536 3 1527.7390 0.9954 0.0120 1 0 1 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P11021|BIP_HUMAN AKFEELNMDLFR AKFEELNM[147]DLFR 764.8768 2 1527.7390 1.0000 0.0001 1 1 0 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|P11021|BIP_HUMAN AKGSETPGATPGSK 644.3305 2 1286.6465 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|O75533|SF3B1_HUMAN AKKPALVAK 463.3133 2 924.6120 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P24534|EF1B_HUMAN AKLAEQAER 508.2802 2 1014.5458 0.9991 0.0010 1 1 0 0.0000 Unknown 0.0000 1 sp|P27348|1433T_HUMAN AKNNTVTPGGKPNK 475.9318 3 1424.7736 0.9896 0.0607 1 1 0 0.0000 Unknown 0.0000 1 sp|P18077|RL35A_HUMAN AKQGYVIYR 366.5415 3 1096.6028 0.9999 0.0005 1 1 0 0.0000 Unknown 0.0000 1 sp|P61313|RL15_HUMAN AKVPAQANGTPTTK 692.3832 2 1382.7518 0.9971 0.0043 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9BQG0|MBB1A_HUMAN ALAAAGYDVEKNNSR 526.9338 3 1577.7797 0.9938 0.0007 1 0 1 0.0000 Unknown 0.0000 1 sp|P10412|H14_HUMAN ALAAFLK 367.2340 2 732.4534 0.9978 0.0164 1 1 0 0.0000 Unknown 0.0000 1 sp|P39019|RS19_HUMAN ALAAFLKK 431.2814 2 860.5483 0.9989 0.0030 1 1 0 0.0000 Unknown 0.0000 1 sp|P39019|RS19_HUMAN ALDAAFYK 449.7370 2 897.4595 0.9952 0.0353 1 1 0 0.0000 Unknown 0.0000 1 sp|P10155|RO60_HUMAN ALDDMISTLK 553.7917 2 1105.5688 0.9990 0.0012 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P49368|TCPG_HUMAN ALDFIASK 432.7449 2 863.4752 1.0000 0.0003 1 1 0 0.0000 Unknown 0.0000 1 sp|O43707|ACTN4_HUMAN ALDIPLVK 434.7788 2 867.5430 0.9901 0.1298 1 1 0 0.0000 Unknown 0.0000 1 sp|Q6P2Q9|PRP8_HUMAN ALDKLDGTEINGR 467.9159 3 1400.7258 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q13247|SRSF6_HUMAN ALDKLDGTEINGR 701.3702 2 1400.7258 1.0000 0.0000 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|Q13247|SRSF6_HUMAN ALDPEISSGEGLR 672.3437 2 1342.6729 1.0000 0.0000 2 2 0 0.0000 Unknown 0.0000 1 sp|Q08554|DSC1_HUMAN ALDTMNFDVIK ALDTM[147]NFDVIK 641.8210 2 1281.6274 1.0000 0.0000 7 5 2 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|P11940|PABP1_HUMAN ALDTMNFDVIK 633.8236 2 1265.6326 1.0000 0.0002 1 1 0 0.0000 Unknown 0.0000 1 sp|P11940|PABP1_HUMAN ALEAVFGK 417.7396 2 833.4647 0.9991 0.0020 1 1 0 0.0000 Unknown 0.0000 1 sp|P38159|RBMX_HUMAN ALEEANADLEVK 651.3327 2 1300.6509 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|O77727|K1C15_SHEEP ALEESNYELEGK 691.3277 2 1380.6409 1.0000 0.0000 2 1 1 0.0000 Unknown 0.0000 1 sp|P13645|K1C10_HUMAN ALEIIPR 406.2554 2 810.4963 0.9925 0.0187 1 1 0 0.0000 Unknown 0.0000 1 sp|P78395|PRAME_HUMAN ALEKIDLK 465.2869 2 928.5593 0.9966 0.0017 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P39023|RL3_HUMAN ALELDPNLYR 602.3219 2 1202.6293 0.9940 0.0459 2 2 0 0.0000 Unknown 0.0000 1 sp|P35580|MYH10_HUMAN ALELTGLK 422.7605 2 843.5065 0.9911 0.0967 1 1 0 0.0000 Unknown 0.0000 1 sp|P19338|NUCL_HUMAN ALEQLNGFELAGRPMK ALEQLNGFELAGRPM[147]K 597.3137 3 1788.9192 1.0000 0.0000 1 1 0 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|Q14498|RBM39_HUMAN ALGISHAK 398.7374 2 795.4603 0.9987 0.0061 1 1 0 0.0000 Unknown 0.0000 1 sp|Q7L2E3|DHX30_HUMAN ALGQNPTNAEVLK 677.8699 2 1353.7252 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P14649|MYL6B_HUMAN ALHHGIDLEK 378.2084 3 1131.6035 0.9983 0.0047 1 1 0 0.0000 Unknown 0.0000 1 sp|P14868|SYDC_HUMAN ALHVNNDR 469.7438 2 937.4730 0.9184 0.8873 1 1 0 0.0000 Unknown 0.0000 1 sp|Q6P2Q9|PRP8_HUMAN ALIAAQYSGAQVR 674.3725 2 1346.7305 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P26641|EF1G_HUMAN ALKDAAEHR 505.7725 2 1009.5305 1.0000 0.0001 2 1 1 0.0000 128.0950:Lys (Addition of lysine due to transpeptidation) 0.0000 1 sp|Q9BYN8|RT26_HUMAN ALKDAAEHR 337.5174 3 1009.5305 0.9989 0.0013 1 0 1 0.0000 128.0950:Lys (Addition of lysine due to transpeptidation) 0.0000 1 sp|Q9BYN8|RT26_HUMAN ALKSEFLVR 531.8189 2 1061.6233 0.9489 0.0073 1 1 0 0.0000 Unknown 0.0000 1 sp|Q00577|PURA_HUMAN ALLAEGVILR 527.8345 2 1053.6545 0.9756 0.0096 1 0 1 0.0000 Unknown 0.0000 1 sp|Q9Y3D9|RT23_HUMAN ALLDQLMGTAR ALLDQLM[147]GTAR 602.8213 2 1203.6281 1.0000 0.0000 3 2 1 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|Q9NQ29|LUC7L_HUMAN ALLEAGALPNAPNSYGR 857.4495 2 1712.8844 1.0000 0.0000 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P42771|CDN2A_HUMAN ALLFIPR 415.2683 2 828.5220 0.9767 0.0741 1 1 0 0.0000 Unknown 0.0000 1 sp|O00585|CCL21_HUMAN ALLFVPR 408.2605 2 814.5064 0.8220 2.3270 1 1 0 0.0000 Unknown 0.0000 1 sp|P07900|HS90A_HUMAN ALLLTGDFSQAGNK 717.8830 2 1433.7515 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9H2W6|RM46_HUMAN ALLNLPGTQTSGEAK 750.4068 2 1498.7991 0.9986 0.0079 1 1 0 0.0000 Unknown 0.0000 1 sp|Q96GM8|TOE1_HUMAN ALLSHDSGSDHLAFVR 431.9733 4 1723.8640 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q7L2E3|DHX30_HUMAN ALPEFFNGK 511.7689 2 1021.5233 0.9986 0.0081 1 1 0 0.0000 Unknown 0.0000 1 sp|Q8TAQ2|SMRC2_HUMAN ALPNNTSSSPQPK 670.8438 2 1339.6731 0.9987 0.0015 2 1 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P04637|P53_HUMAN ALQEFDLALR 588.3245 2 1174.6345 0.9987 0.0055 1 1 0 0.0000 Unknown 0.0000 1 sp|Q96GQ7|DDX27_HUMAN ALQFLEEVK 538.8029 2 1075.5913 0.9987 0.0057 1 1 0 0.0000 Unknown 0.0000 1 sp|P40227|TCPZ_HUMAN ALQKYEK 440.2503 2 878.4861 0.9998 0.0006 1 1 0 0.0000 Unknown 0.0000 1 sp|P48643|TCPE_HUMAN ALQQQQQQQQQK 742.3842 2 1482.7539 0.9992 0.0017 1 1 0 0.0000 Unknown 0.0000 1 sp|Q6Y7W6|GGYF2_HUMAN ALQQVQEK 472.2640 2 942.5135 0.9453 0.6165 1 1 0 0.0000 Unknown 0.0000 1 sp|Q14980|NUMA1_HUMAN ALQSGPPQSR 520.7778 2 1039.5410 1.0000 0.0001 1 1 0 0.0000 Unknown 0.0000 1 sp|O00425|IF2B3_HUMAN ALREQLNRPR 418.2459 3 1251.7158 0.9972 0.0135 1 1 0 0.0000 Unknown 0.0000 1 sp|P82930|RT34_HUMAN ALRTDYNASVSVPDSSGPER 707.6783 3 2120.0132 1.0000 0.0000 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P61978|HNRPK_HUMAN ALRYPMAVGLNK ALRYPM[147]AVGLNK 450.2517 3 1347.7333 1.0000 0.0000 5 4 1 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|Q9Y3U8|RL36_HUMAN ALSSGGSITSPPLSPALPK 890.4961 2 1778.9777 1.0000 0.0000 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|Q6Y7W6|GGYF2_HUMAN ALTAEIVK 422.7605 2 843.5065 0.9289 0.7799 1 1 0 0.0000 Unknown 0.0000 1 sp|P36776|LONM_HUMAN ALTQFPLPK 507.8027 2 1013.5909 0.9999 0.0005 2 2 0 0.0000 Unknown 0.0000 1 sp|Q7L2E3|DHX30_HUMAN ALTQTGGPHVK 554.8090 2 1107.6035 1.0000 0.0003 2 2 0 0.0000 Unknown 0.0000 1 sp|P21333|FLNA_HUMAN ALVATPGKK 442.7818 2 883.5491 0.9989 0.0031 1 1 0 0.0000 Unknown 0.0000 1 sp|P19338|NUCL_HUMAN ALVAYYQK 478.2660 2 954.5175 0.9999 0.0005 1 1 0 0.0000 Unknown 0.0000 1 sp|P62249|RS16_HUMAN ALVDILSEVSK 587.3399 2 1172.6652 0.9988 0.0036 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9BQG0|MBB1A_HUMAN ALVQNDTLLQVK 671.3903 2 1340.7661 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q92522|H1X_HUMAN ALWFQGR 439.2376 2 876.4606 0.8460 1.8380 1 1 0 0.0000 Unknown 0.0000 1 sp|P43243|MATR3_HUMAN AMLDQLMGTSR AMLDQLM[147]GTSR 619.7969 2 1237.5793 0.9981 0.0136 1 1 0 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|Q9Y383|LC7L2_HUMAN AMQRPETAATLKR AM[147]QRPETAATLKR 496.9365 3 1487.7877 1.0000 0.0000 1 1 0 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|P82932|RT06_HUMAN AMTGVEQWPYR AM[147]TGVEQWPYR 677.3164 2 1352.6182 1.0000 0.0000 3 3 0 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|P49368|TCPG_HUMAN AMTGVEQWPYR 669.3189 2 1336.6233 0.9988 0.0037 1 1 0 0.0000 Unknown 0.0000 1 sp|P49368|TCPG_HUMAN AMVYYMSTTIIAAVLGVILVLAIHPGNPK 1019.2411 3 3054.7014 1.0000 0.0000 3 0 3 0.0000 Unknown 0.0000 1 sp|P43004|EAA2_HUMAN ANEFLEVGK 503.7638 2 1005.5131 0.9999 0.0006 1 1 0 0.0000 Unknown 0.0000 1 sp|Q14152|EIF3A_HUMAN ANEFLEVGKK 378.8766 3 1133.6080 0.9966 0.0124 1 1 0 0.0000 Unknown 0.0000 1 sp|Q14152|EIF3A_HUMAN ANEVISK 380.7136 2 759.4127 0.8981 1.1090 1 1 0 0.0000 Unknown 0.0000 1 sp|P50990|TCPQ_HUMAN ANGTTVHVGIHPSK 709.3809 2 1416.7472 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P61254|RL26_HUMAN ANGTTVHVGIHPSK 473.2563 3 1416.7472 1.0000 0.0000 3 0 3 0.0000 -17.0265:Gln->pyro-Glu (Pyro-glu from Q), -17.0265:Ammonia-loss (Loss of ammonia) 0.0000 1 sp|P61254|RL26_HUMAN ANLEESFPR n[43]ANLEESFPR 552.7697 2 1103.5248 0.9965 0.0258 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|Q14690|RRP5_HUMAN ANPALYVLR 508.7980 2 1015.5814 0.9972 0.0095 2 0 2 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|Q6P2Q9|PRP8_HUMAN ANPFGGASHAK 528.7647 2 1055.5148 1.0000 0.0001 1 1 0 0.0000 Unknown 0.0000 1 sp|P62266|RS23_HUMAN ANPFGGASHAK 352.8455 3 1055.5148 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P62266|RS23_HUMAN ANPLSLDSAR 522.2776 2 1042.5406 0.9984 0.0115 1 1 0 0.0000 Unknown 0.0000 1 sp|O14654|IRS4_HUMAN ANQLAEEIESSAQYK 840.9074 2 1679.8002 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q99700|ATX2_HUMAN APAMFNIR APAM[147]FNIR 468.2420 2 934.4694 1.0000 0.0000 5 5 0 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|P61247|RS3A_HUMAN APAMFNIR 460.2445 2 918.4745 1.0000 0.0003 1 1 0 0.0000 Unknown 0.0000 1 sp|P61247|RS3A_HUMAN APAQKAPAPK 489.7901 2 977.5657 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P50914|RL14_HUMAN APAQKVPAQK 346.5416 3 1036.6029 1.0000 0.0000 1 0 1 0.0000 128.0950:Lys (Addition of lysine due to transpeptidation) 0.0000 1 sp|P50914|RL14_HUMAN APAQKVPAQK 519.3087 2 1036.6029 0.8469 0.1420 1 0 1 0.0000 128.0950:Lys (Addition of lysine due to transpeptidation) 0.0000 1 sp|P50914|RL14_HUMAN APEPLSSLK 471.2687 2 940.5229 0.9450 0.6193 1 1 0 0.0000 Unknown 0.0000 1 sp|O75175|CNOT3_HUMAN APGFGDNRK 481.2461 2 960.4777 1.0000 0.0002 1 1 0 0.0000 Unknown 0.0000 1 sp|P10809|CH60_HUMAN APGTPHSHTKPYVR 774.4074 2 1546.8003 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q07020|RL18_HUMAN APGTPHSHTKPYVR 516.6074 3 1546.8003 1.0000 0.0000 4 1 3 0.0000 43.0058:Carbamyl (Carbamylation), 43.0058:Ala->Asn (Ala->Asn substitution) 0.0000 1 sp|Q07020|RL18_HUMAN APGTPHSHTKPYVR 387.7073 4 1546.8003 1.0000 0.0000 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|Q07020|RL18_HUMAN APILIATDVASR 613.8587 2 1225.7029 1.0000 0.0002 1 1 0 0.0000 Unknown 0.0000 1 sp|P17844|DDX5_HUMAN APIQDIWYHEDR 514.9160 3 1541.7263 1.0000 0.0000 2 0 2 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|Q9Y3D9|RT23_HUMAN APQQWATFAR 588.3014 2 1174.5883 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9BYD1|RM13_HUMAN APQVLVLAPTR 582.8585 2 1163.7025 1.0000 0.0000 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|Q9NR30|DDX21_HUMAN APSHSESALNNDSK 728.8367 2 1455.6589 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9NZB2|F120A_HUMAN APSHSESALNNDSK 486.2269 3 1455.6589 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9NZB2|F120A_HUMAN APSSLSDAVPQR 614.3200 2 1226.6254 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q8IX01|SUGP2_HUMAN AQAAAPASVPAQAPK 689.3778 2 1376.7411 1.0000 0.0000 2 1 1 0.0000 Unknown 0.0000 1 sp|P47914|RL29_HUMAN AQAVLEEDHYGMEDVK AQAVLEEDHYGM[147]EDVK 617.2806 3 1848.8199 0.9856 0.0352 1 0 1 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P36776|LONM_HUMAN AQAVSEDAGGNEGR 680.8080 2 1359.6014 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P55884|EIF3B_HUMAN AQDQGEKENPMR n[43]AQDQGEKENPM[147]R 730.8253 2 1459.6361 0.9963 0.0109 2 2 0 0.0000 42.0106:Acetyl (Acetylation), 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|P62913|RL11_HUMAN AQENYEGSEEVSPPQTK 946.9290 2 1891.8434 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q93008|USP9X_HUMAN AQEPESGLSEETQVK 816.3916 2 1630.7686 1.0000 0.0003 1 1 0 0.0000 Unknown 0.0000 1 sp|P78527|PRKDC_HUMAN AQFEGIVTDLIR 681.3748 2 1360.7350 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P38646|GRP75_HUMAN AQFEGIVTDLIRR 506.6193 3 1516.8361 1.0000 0.0000 2 2 0 0.0000 Unknown 0.0000 1 sp|P38646|GRP75_HUMAN AQIHDLVLVGGSTR 489.2755 3 1464.8047 1.0000 0.0000 3 2 1 0.0000 Unknown 0.0000 1 sp|P0DMV8|HS71A_HUMAN AQIHDLVLVGGSTR 733.4096 2 1464.8047 1.0000 0.0000 3 2 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P0DMV8|HS71A_HUMAN AQIHDVVLVGGSTR 484.6036 3 1450.7891 0.9998 0.0010 1 1 0 0.0000 Unknown 0.0000 1 sp|P17066|HSP76_HUMAN AQILPIR n[43]AQILPIR 426.7687 2 851.5229 0.9962 0.0273 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|Q00610|CLH1_HUMAN AQLLQPTLEINPR 746.9277 2 1491.8408 0.9971 0.0214 1 1 0 0.0000 Unknown 0.0000 1 sp|Q12931|TRAP1_HUMAN AQPSASLGVGYR 603.3173 2 1204.6200 0.9988 0.0045 1 1 0 0.0000 Unknown 0.0000 1 sp|Q96PK6|RBM14_HUMAN AQPSVSLGAPYR 623.3329 2 1244.6512 1.0000 0.0003 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|Q96PK6|RBM14_HUMAN AQQNNVEHKVETFSGVYK 693.3482 3 2077.0227 0.9998 0.0002 1 1 0 0.0000 Unknown 0.0000 1 sp|P62081|RS7_HUMAN AQQSLELIQSK 622.8458 2 1243.6771 0.9995 0.0012 1 1 0 0.0000 Unknown 0.0000 1 sp|Q86UP2|KTN1_HUMAN AQSELAAHQKK 404.2228 3 1209.6465 0.9990 0.0032 1 1 0 0.0000 Unknown 0.0000 1 sp|P25786|PSA1_HUMAN AQYDELAR 483.2380 2 964.4614 0.9935 0.0517 1 1 0 0.0000 Unknown 0.0000 1 sp|P05783|K1C18_HUMAN AQYEDIANR 540.2594 2 1078.5042 0.9990 0.0025 1 1 0 0.0000 Unknown 0.0000 1 sp|O95678|K2C75_HUMAN AQYEDIAQK 533.2641 2 1064.5137 0.9998 0.0007 1 1 0 0.0000 Unknown 0.0000 1 sp|P04264|K2C1_HUMAN ARFEELCSDLFR ARFEELCSDLFR 386.4397 4 1541.7296 0.9995 0.0934 1 0 1 0.0000 57.0215:Carbamidomethyl (Iodoacetamide derivative), 57.0215:Gly (Addition of Glycine) Unknown 0.0000 1 sp|P0DMV8|HS71A_HUMAN ARFEELNADLFR 740.8807 2 1479.7468 0.9880 0.0373 1 1 0 0.0000 Unknown 0.0000 1 sp|P11142|HSP7C_HUMAN ARFEELNADLFR 494.2562 3 1479.7468 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P11142|HSP7C_HUMAN ARPATDSFDDYPPR 804.3760 2 1606.7374 0.8980 0.6341 2 0 2 0.0000 Unknown 0.0000 1 sp|P23588|IF4B_HUMAN ARPELEELLAK 634.8640 2 1267.7135 1.0000 0.0001 1 1 0 0.0000 Unknown 0.0000 1 sp|Q96GC5|RM48_HUMAN ARPELEELLAK 423.5784 3 1267.7135 0.9999 0.0001 1 1 0 0.0000 Unknown 0.0000 1 sp|Q96GC5|RM48_HUMAN ASAELIEEEVAK 644.8352 2 1287.6558 1.0000 0.0000 2 2 0 0.0000 Unknown 0.0000 1 sp|P12081|SYHC_HUMAN ASDPGLPAEEPK 605.8011 2 1209.5876 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P27708|PYR1_HUMAN ASDPGLPAEEPKEK 489.9157 3 1466.7252 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P27708|PYR1_HUMAN ASEAKEGEEAGPGDPLLEAVPK 732.0340 3 2193.0801 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q16543|CDC37_HUMAN ASEELQKDLEEVK n[43]ASEELQKDLEEVK 780.3935 2 1558.7725 1.0000 0.0000 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|Q9HB71|CYBP_HUMAN ASESSKPWPDATYGTGSASR 685.6520 3 2053.9341 1.0000 0.0000 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|Q9Y2W1|TR150_HUMAN ASEVMGPVEAAPEYR 803.3825 2 1604.7504 1.0000 0.0002 1 1 0 0.0000 Unknown 0.0000 1 sp|Q8WWY3|PRP31_HUMAN ASEVMGPVEAAPEYR ASEVM[147]GPVEAAPEYR 811.3799 2 1620.7452 1.0000 0.0000 1 1 0 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|Q8WWY3|PRP31_HUMAN ASGADSKGDDLSTAILK n[43]ASGADSKGDDLSTAILK 845.9284 2 1689.8422 1.0000 0.0000 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|P55072|TERA_HUMAN ASGNYATVISHNPETK 844.9156 2 1687.8167 1.0000 0.0000 2 0 2 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P62917|RL8_HUMAN ASGNYATVISHNPETK 563.6128 3 1687.8167 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P62917|RL8_HUMAN ASGPPVSELITK 599.8375 2 1197.6604 1.0000 0.0000 4 3 1 0.0000 Unknown 0.0000 1 sp|P10412|H14_HUMAN ASGVAVSDGVIK n[43]ASGVAVSDGVIK 572.8140 2 1143.6135 1.0000 0.0000 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|P23528|COF1_HUMAN ASINMLR 402.7234 2 803.4323 0.9952 0.0351 1 1 0 0.0000 Unknown 0.0000 1 sp|P18124|RL7_HUMAN ASKEMFEDTVEER n[43]ASKEMFEDTVEER 806.8616 2 1611.7086 0.9986 0.0013 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|Q16576|RBBP7_HUMAN ASLEDAPVDDLTR 701.3464 2 1400.6782 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q7Z2W4|ZCCHV_HUMAN ASLEDAPVDDLTRK 510.5983 3 1528.7732 1.0000 0.0000 1 0 1 0.0000 Unknown 0.0000 1 sp|Q7Z2W4|ZCCHV_HUMAN ASLLKVDQEVK n[43]ASLLKVDQEVK 636.3639 2 1270.7133 1.0000 0.0000 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|P61289|PSME3_HUMAN ASMQQQQQLASAR ASM[147]QQQQQLASAR 731.8569 2 1461.6993 1.0000 0.0000 2 2 0 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|Q9Y3Y2|CHTOP_HUMAN ASMQQQQQLASAR ASM[147]QQQQQLASAR 488.2404 3 1461.6993 0.9995 0.0000 1 0 1 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|Q9Y3Y2|CHTOP_HUMAN ASNVTNK n[43]ASNVTNK 388.2009 2 774.3872 0.9070 1.0100 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|B2RXH8|HNRC2_HUMAN ASNVTNKTDPR n[43]ASNVTNKTDPR 622.8151 2 1243.6156 0.9994 0.0008 2 1 1 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|P07910|HNRPC_HUMAN ASPAGGPLEDVVIER 755.3989 2 1508.7833 1.0000 0.0000 2 1 1 0.0000 Unknown 0.0000 1 sp|Q96AY3|FKB10_HUMAN ASSGLLYPLER 603.3297 2 1204.6449 0.9988 0.0045 1 1 0 0.0000 Unknown 0.0000 1 sp|Q08945|SSRP1_HUMAN ASTNLQDQLEK 623.8173 2 1245.6200 0.9995 0.0012 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9NUL3|STAU2_HUMAN ASYVAPLTAQPATYR 804.9226 2 1607.8307 1.0000 0.0001 3 2 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|Q96PK6|RBM14_HUMAN ATAGDTHLGGEDFDNR 838.3690 2 1674.7234 1.0000 0.0000 4 0 4 0.0000 -71.0371:Gln->Gly (Gln->Gly substitution) 0.0000 1 sp|P0DMV8|HS71A_HUMAN ATAGDTHLGGEDFDNR 559.2484 3 1674.7234 1.0000 0.0000 2 1 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P0DMV8|HS71A_HUMAN ATAPQTQHVSPMR ATAPQTQHVSPM[147]R 480.5735 3 1438.6986 1.0000 0.0000 1 1 0 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|P29692|EF1D_HUMAN ATAVMPDGQFK 582.7895 2 1163.5645 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q06830|PRDX1_HUMAN ATAVVDGAFK 489.7664 2 977.5182 1.0000 0.0000 2 1 1 0.0000 Unknown 0.0000 1 sp|P32119|PRDX2_HUMAN ATAVVDGAFKEVK 667.8693 2 1333.7240 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P32119|PRDX2_HUMAN ATAVVDGAFKEVK 445.5819 3 1333.7240 0.8667 0.1247 1 0 1 0.0000 Unknown 0.0000 1 sp|P32119|PRDX2_HUMAN ATEPAADTGAQPK 628.8095 2 1255.6044 1.0000 0.0001 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9UPT8|ZC3H4_HUMAN ATFDAISK 426.7267 2 851.4389 0.9981 0.0137 1 1 0 0.0000 Unknown 0.0000 1 sp|P15880|RS2_HUMAN ATFIKVPQNQNGK 722.8989 2 1443.7833 0.9940 0.0044 1 0 1 0.0000 43.0058:Carbamyl (Carbamylation), 43.0058:Ala->Asn (Ala->Asn substitution) 0.0000 1 sp|P19338|NUCL_HUMAN ATGDETGAKVER 617.3071 2 1232.5996 1.0000 0.0000 2 1 1 0.0000 Unknown 0.0000 1 sp|P61247|RS3A_HUMAN ATGDETGAKVER 411.8738 3 1232.5996 1.0000 0.0000 3 1 2 0.0000 Unknown 0.0000 1 sp|P61247|RS3A_HUMAN ATGYPLAYVAAK 612.8348 2 1223.6550 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P27708|PYR1_HUMAN ATISNDGATILK 602.3326 2 1202.6506 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q99832|TCPH_HUMAN ATNIEQIFR n[43]ATNIEQIFR 567.3011 2 1132.5876 0.9980 0.0149 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|P18583|SON_HUMAN ATPSENLVPSSAR 664.8438 2 1327.6730 0.9933 0.0543 1 1 0 0.0000 Unknown 0.0000 1 sp|Q8N684|CPSF7_HUMAN ATVEELKEK 523.7900 2 1045.5654 0.9862 0.0443 1 1 0 0.0000 Unknown 0.0000 1 sp|O95232|LC7L3_HUMAN ATVEQVLDPR 564.3063 2 1126.5981 0.9998 0.0007 2 1 1 0.0000 Unknown 0.0000 1 sp|Q9BRS2|RIOK1_HUMAN ATVVESSEK 475.2455 2 948.4764 0.9998 0.0008 2 2 0 0.0000 Unknown 0.0000 1 sp|P61981|1433G_HUMAN AVAAPRPDR 476.7698 2 951.5250 0.9050 1.0320 1 1 0 0.0000 Unknown 0.0000 1 sp|O75534|CSDE1_HUMAN AVAGWEEVLR 565.3036 2 1128.5927 1.0000 0.0002 2 2 0 0.0000 Unknown 0.0000 1 sp|Q7L2E3|DHX30_HUMAN AVANQTSATFLR 639.8436 2 1277.6727 1.0000 0.0000 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P62191|PRS4_HUMAN AVAQALEVIPR 583.8482 2 1165.6818 1.0000 0.0000 2 2 0 0.0000 Unknown 0.0000 1 sp|P49368|TCPG_HUMAN AVASDFYLR n[43]AVASDFYLR 542.2771 2 1082.5397 0.9945 0.0413 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|Q96A72|MGN2_HUMAN AVAVVVDPIQSVK 662.8953 2 1323.7760 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|O00487|PSDE_HUMAN AVDDGVNTFK 533.2641 2 1064.5137 1.0000 0.0000 3 2 1 0.0000 Unknown 0.0000 1 sp|P50990|TCPQ_HUMAN AVDEMNGKELNGK AVDEM[147]NGKELNGK 474.2294 3 1419.6664 1.0000 0.0000 3 3 0 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|P11940|PABP1_HUMAN AVDFAER 404.2034 2 806.3923 0.9923 0.0694 1 1 0 0.0000 Unknown 0.0000 1 sp|P62917|RL8_HUMAN AVDIPHMDIEALK 484.5903 3 1450.7490 0.9794 0.1132 1 1 0 0.0000 Unknown 0.0000 1 sp|P62906|RL10A_HUMAN AVDIPHMDIEALK AVDIPHM[147]DIEALK 489.9219 3 1466.7439 1.0000 0.0000 1 1 0 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|P62906|RL10A_HUMAN AVDIPHMDIEALKK 395.7183 4 1578.8440 1.0000 0.0000 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P62906|RL10A_HUMAN AVDIPHMDIEALKK AVDIPHM[147]DIEALKK 532.6202 3 1594.8389 1.0000 0.0000 1 0 1 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P62906|RL10A_HUMAN AVDIPHMDIEALKK AVDIPHM[147]DIEALKK 399.7170 4 1594.8389 1.0000 0.0006 2 0 2 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P62906|RL10A_HUMAN AVDPEDDFQR 596.2674 2 1190.5203 1.0000 0.0000 2 1 1 0.0000 Unknown 0.0000 1 sp|Q99848|EBP2_HUMAN AVDSLVPIGR 513.8007 2 1025.5868 1.0000 0.0001 1 1 0 0.0000 Unknown 0.0000 1 sp|P25705|ATPA_HUMAN AVDSPNIK 422.2322 2 842.4498 0.9988 0.0036 2 2 0 0.0000 0.0364:Gln->Lys (Gln->Lys substitution) 0.0000 1 sp|Q7L2E3|DHX30_HUMAN AVDSQILPK 485.7820 2 969.5494 1.0000 0.0004 2 2 0 0.0000 Unknown 0.0000 1 sp|Q02878|RL6_HUMAN AVFPSIVGRPR 599.8563 2 1197.6981 0.9924 0.0663 1 1 0 0.0000 Unknown 0.0000 1 sp|A5A3E0|POTEF_HUMAN AVFVDLEPTVIDEVR 851.4564 2 1700.8983 0.9915 0.0858 1 1 0 0.0000 Unknown 0.0000 1 sp|P68363|TBA1B_HUMAN AVLKFAAATGATPIAGR 807.9699 2 1613.9253 0.9815 0.0710 1 1 0 0.0000 Unknown 0.0000 1 sp|P08865|RSSA_HUMAN AVLLAGPPGTGK 540.8242 2 1079.6338 1.0000 0.0001 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9Y265|RUVB1_HUMAN AVLLDLEPR 513.3031 2 1024.5916 0.9988 0.0042 2 1 1 0.0000 Unknown 0.0000 1 sp|P23258|TBG1_HUMAN AVLVDLEPGTMDSVR AVLVDLEPGTM[147]DSVR 809.4111 2 1616.8077 1.0000 0.0002 6 5 1 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|P04350|TBB4A_HUMAN AVLVDLEPGTMDSVR 801.4137 2 1600.8129 0.9939 0.0473 1 1 0 0.0000 Unknown 0.0000 1 sp|P04350|TBB4A_HUMAN AVNSATGVPTV 508.2745 2 1014.5345 0.9989 0.0029 1 1 0 0.0000 Unknown 0.0000 1 sp|P11940|PABP1_HUMAN AVQELVHPVVDR 681.3804 2 1360.7462 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|A5YKK6|CNOT1_HUMAN AVQISKK n[43]AVQISKK 408.2529 2 814.4913 0.8160 2.4770 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|P23396|RS3_HUMAN AVSREDSVKPGAHLTVK 598.6671 3 1792.9796 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P51991|ROA3_HUMAN AVSVPASFFR 540.7955 2 1079.5764 0.9970 0.0218 2 2 0 0.0000 Unknown 0.0000 1 sp|O14654|IRS4_HUMAN AVSVTPIRDTK n[43]AVSVTPIRDTK 614.8484 2 1227.6823 1.0000 0.0000 1 1 0 0.0000 42.0106:Acetyl (Acetylation) Unknown 0.0000 1 sp|Q9NR56|MBNL1_HUMAN AVTELNEPLSNEDR 793.8864 2 1585.7583 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q04917|1433F_HUMAN AVTEMNGR 439.2135 2 876.4124 0.9964 0.0259 1 1 0 0.0000 Unknown 0.0000 1 sp|P11940|PABP1_HUMAN AVTEQGAELSNEER 766.8630 2 1531.7114 1.0000 0.0000 2 1 1 0.0000 43.0058:Carbamyl (Carbamylation), 43.0058:Ala->Asn (Ala->Asn substitution) 0.0000 1 sp|P27348|1433T_HUMAN AVTEQGHELSNEER 533.5850 3 1597.7332 1.0000 0.0000 3 1 2 0.0000 128.0950:Lys (Addition of lysine due to transpeptidation) 0.0000 1 sp|P31946|1433B_HUMAN AVTEQGHELSNEER 799.8739 2 1597.7332 1.0000 0.0000 3 1 2 0.0000 128.0950:Lys (Addition of lysine due to transpeptidation) 0.0000 1 sp|P31946|1433B_HUMAN AVTIFIR 410.2580 2 818.5014 0.9931 0.0560 1 1 0 0.0000 Unknown 0.0000 1 sp|P48643|TCPE_HUMAN AVTPVPTKTEEVSNLK 571.6524 3 1711.9355 1.0000 0.0000 1 0 1 0.0000 Unknown 0.0000 1 sp|Q6PKG0|LARP1_HUMAN AVTPVPTKTEEVSNLK 856.9750 2 1711.9355 0.9824 0.0648 2 1 1 0.0000 Unknown 0.0000 1 sp|Q6PKG0|LARP1_HUMAN AVVGVVAGGGR 471.2800 2 940.5455 1.0000 0.0000 2 1 1 0.0000 Unknown 0.0000 1 sp|P62917|RL8_HUMAN AVVGVVAGGGRIDKPILK 583.6964 3 1748.0674 1.0000 0.0000 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P62917|RL8_HUMAN AVVGVVAGGGRIDKPILK 438.0241 4 1748.0674 0.9998 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P62917|RL8_HUMAN AVVPSKR 378.7400 2 755.4654 0.9786 0.0624 1 1 0 0.0000 Unknown 0.0000 1 sp|B2RXH8|HNRC2_HUMAN AVVVVDDR 436.7454 2 871.4763 0.9907 0.1094 1 1 0 0.0000 Unknown 0.0000 1 sp|Q8WXF1|PSPC1_HUMAN AWEEYYK 494.7242 2 987.4338 0.9982 0.0131 1 1 0 0.0000 Unknown 0.0000 1 sp|Q92945|FUBP2_HUMAN AWEEYYKK 558.7716 2 1115.5287 0.9992 0.0017 1 1 0 0.0000 Unknown 0.0000 1 sp|Q92945|FUBP2_HUMAN AWGPGLEGGVVGK 613.8300 2 1225.6455 1.0000 0.0001 1 1 0 0.0000 Unknown 0.0000 1 sp|P21333|FLNA_HUMAN AWNDIKK 437.7427 2 873.4709 0.9982 0.0130 1 1 0 0.0000 Unknown 0.0000 1 sp|P36578|RL4_HUMAN AYEKPPEKK 545.3005 2 1088.5864 1.0000 0.0001 1 1 0 0.0000 Unknown 0.0000 1 sp|Q12905|ILF2_HUMAN AYGELPEHAK 557.7799 2 1113.5453 1.0000 0.0001 2 2 0 0.0000 Unknown 0.0000 1 sp|O14602|IF1AY_HUMAN AYILNLVK 467.2920 2 932.5695 0.9913 0.0919 1 1 0 0.0000 Unknown 0.0000 1 sp|P50991|TCPD_HUMAN AYLGDTSEAKEFAK 765.3777 2 1528.7408 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q6Y7W6|GGYF2_HUMAN AYLGDTSEAKEFAK 510.5875 3 1528.7408 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q6Y7W6|GGYF2_HUMAN AYLVPENFR 554.7929 2 1107.5712 0.9979 0.0155 1 1 0 0.0000 Unknown 0.0000 1 sp|Q86UK7|ZN598_HUMAN AYQKQPTIFQNK 733.3935 2 1464.7725 0.9949 0.0027 1 0 1 0.0000 43.0058:Ala->Asn (Ala->Asn substitution), 43.0058:Carbamyl (Carbamylation) 0.0000 1 sp|P62280|RS11_HUMAN AYSEAHEISK 378.8523 3 1133.5352 0.9992 0.0027 1 1 0 0.0000 Unknown 0.0000 1 sp|P61981|1433G_HUMAN AYSEAHEISKEHMQPTHPIR AYSEAHEISKEHM[147]QPTHPIR 793.0499 3 2376.1279 1.0000 0.0000 1 0 1 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P61981|1433G_HUMAN AYSEAHEISKEHMQPTHPIR AYSEAHEISKEHM[147]QPTHPIR 595.0392 4 2376.1279 1.0000 0.0015 1 0 1 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|P61981|1433G_HUMAN AYTPPEDLQSR 638.8120 2 1275.6094 0.9991 0.0021 1 1 0 0.0000 Unknown 0.0000 1 sp|Q8N5N7|RM50_HUMAN CEALAGAPLDNAPK CEALAGAPLDNAPK 713.8533 2 1425.6921 1.0000 0.0001 4 0 4 0.0000 57.0215:Gly (Addition of Glycine), 57.0215:Carbamidomethyl (Iodoacetamide derivative) -9.0327:Arg->Phe (Arg->Phe substitution) 0.0000 1 sp|P52815|RM12_HUMAN CGYPAKR CGYPAKR 284.4779 3 850.4119 0.8592 0.1591 1 0 1 0.0000 57.0215:Carbamidomethyl (Iodoacetamide derivative), 57.0215:Gly (Addition of Glycine) Unknown 0.0000 1 sp|P61927|RL37_HUMAN CQFAHGLGELR CQFAHGLGELR 429.8802 3 1286.6189 0.9949 0.0003 6 0 6 0.0000 57.0215:Carbamidomethyl (Iodoacetamide derivative), 57.0215:Gly (Addition of Glycine) Unknown 0.0000 1 sp|P26651|TTP_HUMAN CQFAHGLGELR CQFAHGLGELR 322.6620 4 1286.6189 1.0000 0.0905 8 0 8 0.0000 57.0215:Carbamidomethyl (Iodoacetamide derivative), 57.0215:Gly (Addition of Glycine) Unknown 0.0000 1 sp|P26651|TTP_HUMAN CQFAHGLGELR CQFAHGLGELR 644.3167 2 1286.6189 0.9998 0.0002 4 0 4 0.0000 57.0215:Gly (Addition of Glycine), 57.0215:Carbamidomethyl (Iodoacetamide derivative) -57.0215:Gln->Ala (Gln->Ala substitution), -57.0215:Asn->Gly (Asn->Gly substitution) 0.0000 1 sp|P26651|TTP_HUMAN DAAGSGDKPGADTGR 687.8158 2 1373.6171 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q96EL3|RM53_HUMAN DAAGSGDKPGADTGR 458.8796 3 1373.6171 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q96EL3|RM53_HUMAN DAASKPSGEGSFSKPGDGGSPSKPSDHEPPKNK 820.6431 4 3278.5435 1.0000 0.0000 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|O14654|IRS4_HUMAN DAASVDKVLELK 644.3613 2 1286.7081 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|O14979|HNRDL_HUMAN DAEDAMDAMDGAVLDGR DAEDAM[147]DAM[147]DGAVLDGR 892.3590 2 1782.7035 1.0000 0.0000 1 0 1 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|Q01130|SRSF2_HUMAN DAEDAMDAMDGAVLDGR DAEDAM[147]DAMDGAVLDGR 884.3616 2 1766.7086 1.0000 0.0000 3 3 0 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|Q01130|SRSF2_HUMAN DAEIPEGAAR 514.7539 2 1027.4933 0.9989 0.0031 1 1 0 0.0000 Unknown 0.0000 1 sp|O14654|IRS4_HUMAN DAFADAVQR 496.7434 2 991.4722 0.9988 0.0048 1 1 0 0.0000 Unknown 0.0000 1 sp|Q92945|FUBP2_HUMAN DAFTDSAISAK 563.2747 2 1124.5348 1.0000 0.0001 2 2 0 0.0000 Unknown 0.0000 1 sp|Q99700|ATX2_HUMAN DAGTIAGLNVLR 600.3407 2 1198.6669 1.0000 0.0001 1 1 0 0.0000 Unknown 0.0000 1 sp|P11142|HSP7C_HUMAN DAGVIAGLNVLR 599.3511 2 1196.6876 0.9995 0.0011 2 2 0 0.0000 Unknown 0.0000 1 sp|P0DMV8|HS71A_HUMAN DANNGNLQLR 557.7836 2 1113.5526 1.0000 0.0001 1 1 0 0.0000 Unknown 0.0000 1 sp|P05455|LA_HUMAN DAQGQPGLER 535.7648 2 1069.5151 0.8599 1.6110 1 1 0 0.0000 -0.0364:Lys->Gln (Lys->Gln substitution) 0.0000 1 sp|O15027|SC16A_HUMAN DATGAEEEAPWAATER 852.3790 2 1702.7434 0.9999 0.0004 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|Q8IVS2|FABD_HUMAN DAVEDLESVGK 581.2853 2 1160.5560 1.0000 0.0002 1 1 0 0.0000 Unknown 0.0000 1 sp|P81605|DCD_HUMAN DAVEQQGEVKK 410.8823 3 1229.6251 0.9422 0.0003 1 0 1 0.0000 Unknown 0.0000 1 sp|Q9NX58|LYAR_HUMAN DAVSNTTNQLESK 703.8415 2 1405.6685 1.0000 0.0000 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|Q86UP2|KTN1_HUMAN DDDSLPAETGQNHPFFRR 526.2476 4 2100.9612 0.9998 2.0550 1 0 1 0.0000 Unknown 0.0000 1 sp|O95071|UBR5_HUMAN DDKDAIEEK 266.3791 4 1061.4875 0.8582 0.0253 1 0 1 0.0000 Unknown 0.0000 1 sp|P36776|LONM_HUMAN DDKHGSYEDAVHSGALND 643.9452 3 1928.8137 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P17987|TCPA_HUMAN DDWEEKR 489.2198 2 976.4250 0.8399 1.9500 1 1 0 0.0000 Unknown 0.0000 1 sp|P43243|MATR3_HUMAN DDYSRDDYR 602.7468 2 1203.4791 0.9967 0.0083 1 1 0 0.0000 Unknown 0.0000 1 sp|P23588|IF4B_HUMAN DDYTIPDEYR 429.5226 3 1285.5460 0.9996 0.0893 1 0 1 0.0000 Unknown 0.0000 1 sp|P43243|MATR3_HUMAN DEILPTTPISEQK 735.8878 2 1469.7611 0.9989 0.0032 2 2 0 0.0000 Unknown 0.0000 1 sp|P23396|RS3_HUMAN DETEFYLGK 551.2585 2 1100.5024 0.9988 0.0040 1 1 0 0.0000 Unknown 0.0000 1 sp|P18077|RL35A_HUMAN DETEFYLGKR 419.8751 3 1256.6035 0.9998 0.0001 1 1 0 0.0000 Unknown 0.0000 1 sp|P18077|RL35A_HUMAN DETEFYLGKR 629.3090 2 1256.6035 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P18077|RL35A_HUMAN DETNYGIPQR 596.7832 2 1191.5519 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P63244|RACK1_HUMAN DFIDLTLSK 526.2871 2 1050.5596 0.9899 0.1374 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9BZE4|NOG1_HUMAN DFVEAPPPK 500.2609 2 998.5072 0.9989 0.0032 2 2 0 0.0000 Unknown 0.0000 1 sp|Q6PKG0|LARP1_HUMAN DFVNYLVR 513.2743 2 1024.5341 0.9944 0.0426 1 1 0 0.0000 Unknown 0.0000 1 sp|Q08211|DHX9_HUMAN DGGQTESNEEGKENR 550.5714 3 1648.6924 0.9997 0.0002 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|Q14157|UBP2L_HUMAN DGGSGNSTIIVSR 631.8203 2 1261.6261 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q7Z6Z7|HUWE1_HUMAN DGKLVSESSDVLPK 737.3934 2 1472.7722 0.9461 0.3234 1 1 0 0.0000 Unknown 0.0000 1 sp|P05787|K2C8_HUMAN DGLGSDNIGSR 545.7597 2 1089.5049 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P98175|RBM10_HUMAN DGPPHQVLVVPLHSR 550.9739 3 1649.9000 0.9947 0.0221 2 2 0 0.0000 Unknown 0.0000 1 sp|Q2NL82|TSR1_HUMAN DGPVRPQNAEEEKR 542.2727 3 1623.7964 1.0000 0.0000 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|Q92504|S39A7_HUMAN DGQGTAGGHGSGGGQRPGGGHGSGGGQGPGDGHGSGGGK 803.3502 4 3209.3716 0.8500 0.1068 1 0 1 0.0000 2.0042:Label:18O(1) (O18 Labeling), 1.9979:Glu->Met (Glu->Met substitution) 0.0000 1 sp|O14654|IRS4_HUMAN DGQLLIR 407.7427 2 813.4708 0.9447 0.6230 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9HCE1|MOV10_HUMAN DGQVINETSQHHDDLE 612.9381 3 1835.7924 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P08670|VIME_HUMAN DGQVINETSQHHDDLE 918.9035 2 1835.7924 1.0000 0.0002 2 1 1 0.0000 Unknown 0.0000 1 sp|P08670|VIME_HUMAN DGSDVIYPAR 546.7696 2 1091.5247 1.0000 0.0000 2 1 1 0.0000 Unknown 0.0000 1 sp|P25685|DNJB1_HUMAN DGSLASNPYSGDLTK 762.8624 2 1523.7103 0.9932 0.0261 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|Q14157|UBP2L_HUMAN DGTGVVEFVR 539.7800 2 1077.5454 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q07955|SRSF1_HUMAN DGTGVVEFVRK 402.8874 3 1205.6404 0.9971 0.0093 1 1 0 0.0000 Unknown 0.0000 1 sp|Q07955|SRSF1_HUMAN DGVPEGAQLQGPVHR 780.3998 2 1558.7850 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|P16989|YBOX3_HUMAN DGYDYDGYR 562.2199 2 1122.4253 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q07955|SRSF1_HUMAN DHHQPLGR 480.2439 2 958.4733 0.9944 0.0421 1 1 0 0.0000 Unknown 0.0000 1 sp|P46087|NOP2_HUMAN DHIQNGKLDLTK 346.1913 4 1380.7361 1.0000 0.0000 2 0 2 0.0000 Unknown 0.0000 1 sp|Q9NR30|DDX21_HUMAN DHIQNGKLDLTK 461.2526 3 1380.7361 1.0000 0.0000 1 0 1 0.0000 Unknown 0.0000 1 sp|Q9NR30|DDX21_HUMAN DHNVEFR 458.7172 2 915.4199 0.9939 0.0466 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9NWU5|RM22_HUMAN DHQYQFLEDAVR 507.5757 3 1519.7054 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q13263|TIF1B_HUMAN DIDEVSSLLR 573.8037 2 1145.5928 0.9988 0.0037 1 1 0 0.0000 Unknown 0.0000 1 sp|P50990|TCPQ_HUMAN DIEDVFYK 514.7503 2 1027.4861 0.9169 0.9036 1 1 0 0.0000 Unknown 0.0000 1 sp|Q07955|SRSF1_HUMAN DIGFIKLD 460.7580 2 919.5014 0.9832 0.0597 1 1 0 0.0000 Unknown 0.0000 1 sp|P62273|RS29_HUMAN DIIALNPLYR 594.3427 2 1186.6708 0.9999 0.0005 1 1 0 0.0000 Unknown 0.0000 1 sp|P36776|LONM_HUMAN DIIHDPGR 461.7406 2 921.4667 0.9904 0.1218 1 1 0 0.0000 Unknown 0.0000 1 sp|P62917|RL8_HUMAN DIIHDPGRGAPLAK 365.7058 4 1458.7942 1.0000 0.0046 1 0 1 0.0000 Unknown 0.0000 1 sp|P62917|RL8_HUMAN DIPGLTDTTVPR 642.8433 2 1283.6720 0.9991 0.0020 1 1 0 0.0000 Unknown 0.0000 1 sp|P62753|RS6_HUMAN DISLSDYK 470.7347 2 939.4549 0.9905 0.1159 1 1 0 0.0000 Unknown 0.0000 1 sp|Q06830|PRDX1_HUMAN DISTTLNADEAVAR 738.3703 2 1474.7261 1.0000 0.0001 2 1 1 0.0000 Unknown 0.0000 1 sp|O95757|HS74L_HUMAN DIVENYFMR DIVENYFM[147]R 601.7791 2 1201.5436 0.8386 1.0010 1 1 0 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|Q13263|TIF1B_HUMAN DKGFGFIR 470.2559 2 938.4973 0.9950 0.0157 1 1 0 0.0000 Unknown 0.0000 1 sp|Q15233|NONO_HUMAN DKITRPGAK 493.2931 2 984.5716 0.9991 0.0010 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9UBS4|DJB11_HUMAN DKLEEILR 508.2927 2 1014.5709 0.9970 0.0049 1 1 0 0.0000 Unknown 0.0000 1 sp|O15042|SR140_HUMAN DKLNNLVLFDK 440.2503 3 1317.7291 0.9990 0.0039 1 1 0 0.0000 Unknown 0.0000 1 sp|P62851|RS25_HUMAN DKLNNLVLFDK 659.8718 2 1317.7291 1.0000 0.0000 3 1 2 0.0000 Unknown 0.0000 1 sp|P62851|RS25_HUMAN DKTIIMWK DKTIIM[147]WK 525.7862 2 1049.5579 0.9876 0.0388 1 1 0 0.0000 15.9949:Oxidation (Oxidation or Hydroxylation) Unknown 0.0000 1 sp|P63244|RACK1_HUMAN DKVQAGDVITIDK 701.3828 2 1400.7511 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9Y230|RUVB2_HUMAN DKVQAGDVITIDK 467.9243 3 1400.7511 1.0000 0.0000 1 0 1 0.0000 0.9970:Label:15N(1) (15N(1)) 0.0000 1 sp|Q9Y230|RUVB2_HUMAN DLAEITTLDR 573.8036 2 1145.5927 0.9992 0.0019 2 2 0 0.0000 Unknown 0.0000 1 sp|Q9BS26|ERP44_HUMAN DLAGSIIGK 437.2556 2 872.4967 0.8480 1.8040 1 1 0 0.0000 Unknown 0.0000 1 sp|P61978|HNRPK_HUMAN DLDELSRYPEDK 740.3517 2 1478.6888 0.9997 0.0005 1 0 1 0.0000 43.0058:Carbamyl (Carbamylation), 43.0170:Xle->Arg (Leu/Ile->Arg substitution), 43.0058:Ala->Asn (Ala->Asn substitution) 0.0000 1 sp|P43243|MATR3_HUMAN DLDELSRYPEDK 493.9035 3 1478.6888 0.9998 0.0002 1 1 0 0.0000 Unknown 0.0000 1 sp|P43243|MATR3_HUMAN DLEAEHVEVEDTTLNR 935.4449 2 1868.8752 0.9903 0.1246 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9H3K6|BOLA2_HUMAN DLEAEHVEVEDTTLNR 623.9657 3 1868.8752 1.0000 0.0000 1 1 0 0.0000 Unknown 0.0000 1 sp|Q9H3K6|BOLA2_HUMAN DLEEFFSTVGK 636.3113 2 1270.6080 0.9990 0.0026 1 1 0 0.0000 Unknown 0.0000 1 sp|Q14498|RBM39_HUMAN DLEPGEVPSDSDEDGEHK 489.4592 4 1953.8076 1.0000 3.5440 1 0 1 0.0000 Unknown 0.0000 1 sp|Q96T58|MINT_HUMAN DLIDNSFNR 547.2672 2 1092.5199 0.9989 0.0029 2 2 0 0.0000 Unknown 0.0000 1 sp|Q8IY81|SPB1_HUMAN DLILADYGKK 568.3215 2 1134.6284 1.0000 0.0002 1 1 0 0.0000 Unknown 0.0000 1 sp|Q6P2Q9|PRP8_HUMAN DLILNPR 420.7505 2 839.4864 0.9029 1.0550 1 1 0 0.0000 Unknown 0.0000 1 sp|Q15393|SF3B3_HUMAN DLKDYFTK 515.2662 2 1028.5178 0.9997 0.0006 1 1 0 0.0000 Unknown 0.0000 1 sp|Q99729|ROAA_HUMAN DLLGENFQK 532.2745 2 1062.5344 0.8313 2.1210 1 1 0 0.0000 Unknown 0.0000 1 sp|Q86UK7|ZN598_HUMAN DLLHPSLEEEK 655.3353 2 1308.6560 1.0000 0.0002 2 2 0 0.0000 Unknown 0.0000 1 sp|Q71UM5|RS27L_HUMAN DLLHPSPEEEK 647.3197 2 1292.6248 0.9746 0.0038 1 0 1 0.0000 1.9979:Glu->Met (Glu->Met substitution), 2.0042:Label:18O(1) (O18 Labeling), 1.9941:Label:15N(2) (15N(2)) 0.0000 1 sp|P42677|RS27_HUMAN DLLHPSPEEEK 431.8822 3 1292.6248 1.0000 0.0000 1 0 1 0.0000 0.9970:Label:15N(1) (15N(

andytyk commented 6 years ago

Turns out the philosopher that is used to process the MSFragger output is currently only restricted to the -500 to 500 range (you should still see your crosslinked peptides in the pepXML output but they are not being correctly summarized in the philosopher reports). We will be releasing an updated version of philosopher which does not have this limitation in a few days.

ThomasGossenreiter commented 6 years ago

Great :+1: Thank you!

prvst commented 6 years ago

@ThomasGossenreiter

Just a clarification; Philospher will process all the results from your search, regardless of the delta mass. The current restriction of 500 Da is only applied when Philosopher tries to map the modification mass to UniMod. This restriction also affects the mass binning, so you will not see those PSMs on the histogram. If you open your PSM table you should see all those observations there, independently of the mass difference.

ThomasGossenreiter commented 6 years ago

@prvst thank you for the information, with the PSM table I can prepare the histogram myself now. very nice! Thank you!

chhh commented 6 years ago

@ThomasGossenreiter Hi Thomas, if you're after histograms and don't mind test driving some software, shoot me an email at dmitriya at umich edu, I'll send you our pre-release version of a tool made specifically for that purpose.

chhh commented 6 years ago

Resolved and contacted by mail