Closed anfoss closed 2 years ago
The log says that the file is corrupted. Please check your computer's file system and files.
Best,
Fengchao
As I wrote above the file can be searched in MSfragger with the same pipeline i.e if I remove all the other files and load it again I can run the entire pipeline.
The file system issue normally hard to reproduce. Can you try it again with and without many files?
That is exactly what I am reporting that this MS file can be run individually without issues while with other file it loads and fails.
As you can see from the log, MSFragger loads each file sequentially. There are many reasons causing MSFragger failed to load the file. Mostly due the the issue of your disk (the log also said that "database disk image is malformed"). If you think there is something that we should fix, can you send your files to us?
Best,
Fengchao
I understand that MSfragger loads each file sequentially but if it would be an issue from our end (like a thermo corrupted file) I will not be able to search it in any search engine/open it.
I can convert this file, search it within Fragpipe and MaxQuant, convert it using MSconvert and open it using data analysis in Hystar, so the file is clearly fine, what would be the reason that when loaded with other file makes it corrupted?
There are many reasons causing MSFragger failed to load the file. Mostly due the the issue of your disk (the log also said that "database disk image is malformed").
It could be your file system's issue which causes MSFragger failed to load the file at that moment. Can you try to reproduce the error? If you can reproduce it, can you remove all other .d and try again without changing any other settings?
Again, if you think there is something that we should fix, can you send all of your files to us because we cannot reproduce it with our own files?
Best,
Fengchao
I wrote this when I opened the issue, I can search the file without changing any other setting by just re-loading it alone and everything runs.
Is there an email I can use to share the files?
Can you try again with all 30 files?
Here (https://www.dropbox.com/sh/ze66kwto1iwuatg/AABfcfHqjilAwWFO3XFwKVsza?dl=0) is the link to upload the files.
Best,
Fengchao
Figured it out, seems that if I assign names automatically with 'by filename' in the main msfragger tab it fails while if I do it sequentially or add them by hand it does not.
Although I don't think assigning experimental names by filename would cause any issue, I am glad that you figured it out.
Best,
Fengchao
Describe the bug
I am searching 30 DDA-PASEF file with LFQ-MBR but Fragpipe fails with the error : sumich.ms.fileio.exceptions.FileParsingException: umich.ms.fileio.filetypes.bruker.BrukerException: Error reading PASEF spectra with tims_read_pasef_msms_for_frame_v2(): SQLITE_CORRUPT[11]: database disk image is malformed
The file which cause the error can be searched as standalone file with the same pipeline without this error (either by using the MZbin or the .d).
I am attaching the log file log_2022-05-01_18-46-50.txt