Nesvilab / MSFragger

Ultrafast, comprehensive peptide identification for mass spectrometry–based proteomics
https://msfragger.nesvilab.org
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Everything looks fine but Philosopher reports "No PSM was found in data set." #43

Closed Golden-proteogenomics closed 4 years ago

Golden-proteogenomics commented 4 years ago

Hello,

Thank you for your support and help with the MSFragger user. I have completed several data search work, but there is still a problem that is described as follow: “INFO[12:45:11] Executing Filter v1.5.1
INFO[12:45:11] Processing peptide identification files
FATA[12:45:47] No PSM was found in data set.
Process 'ReportFilter' finished, exit code: 1

Process returned non-zero exit code, stopping” log_2019-10-12_16-05-43.txt

Attachment is the log file for this running and . The software version I use are MSFragger-20190628.jar and the FragPipe v9.4.

anesvi commented 4 years ago

I think you should upgrade to the latest versions of the tools and rerun before we can help

There were many bug fixes

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On Oct 12, 2019, at 4:10 AM, Golden-proteogenomics notifications@github.com wrote:

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Hello,

Thank you for your support and help with the MSFragger user. I have completed several data search work, but there is still a problem that is described as follow: “INFO[12:45:11] Executing Filter v1.5.1 INFO[12:45:11] Processing peptide identification files FATA[12:45:47] No PSM was found in data set. Process 'ReportFilter' finished, exit code: 1

Process returned non-zero exit code, stopping” log_2019-10-12_16-05-43.txthttps://github.com/Nesvilab/MSFragger/files/3720355/log_2019-10-12_16-05-43.txt

Attachment is the log file for this running and . The software version I use are MSFragger-20190628.jar and the FragPipe v9.4.

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prvst commented 4 years ago

Try the following MSFragger parameters using the exact syntax (i.e. use gluc, not GluC), and run again.

search_enzyme_name = gluc
search_enzyme_cutafter = DE
search_enzyme_butnotafter = P
fcyu commented 4 years ago

It was due to one or more interact-*.pep.xml having no PSM. Has been fixed.