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NinaGXie / SADDLE

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I can't find the readme file #2

Open kf-wei opened 2 years ago

kf-wei commented 2 years ago

Hello,

" For details about running the code and interpreting the results, please refer to the "README.txt" inside the folder."

I can't find the readme file which tell to use the code.

I didn't use matlab before ,so I can't run it without instruction.

Could you please provide some description about how to use this tool ?

I have primers and want to use SADDLE to check it.

Thank you very much.

NinaGXie commented 2 years ago

Please find updated README file in the NGS analysis folder.

Please download Matlab to run the code. I would recommend using R2021b (what we used) to run the code. However, using other versions should be fine from our past experience.

The analysis code is used for the analysis of dimer species from NGS data.

kf-wei commented 2 years ago

Hi Nina,

Thank you for your help.

SADDLE can design and pick primers for target genes. The NGS_analysis code maybe not what I need.

"The MATLAB code used for multiplex PCR primer algorithm is available upon request under NDA for academic lab. "

Is there any way to request the code for SADDLE with complete funtion ?

jan-glx commented 2 years ago

Oh lol, @kf-wei , stay away (three red flags (MATLAB, "upon request", Nat. Com.), one should be enough)! I am currently on the lookout for a multiplex primer design tool, could you share what you end up using?

[Sorry, @NinaGXie , probably (mostly) not your fault.]