NorwegianVeterinaryInstitute / DemultiplexRawSequenceData

A workflow automation script: demultiplex the library sequence, run quality checks, deliver to archiving and processing afterwards
GNU General Public License v3.0
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feature request: detect when a run needs to be demultiplexed as single lane #79

Open georgemarselis-nvi opened 1 year ago

georgemarselis-nvi commented 1 year ago

there is at least one run that needs to be demultiplexed as a single lane

alter the bcl2fast() method to catch a failure, check if bcl2fastq has complained appropriately and restart mutliplexing with appropriate command line.

Things that need to be figured out:

What was the relevant RunID: What was the altered command line: What was the difference with regular command line:

from a quick investigation:

find . -iname 02_demultiplex.log -exec grep -i '0 error' {} \;

find . -iname 02_demultiplex.log -exec grep -inl '1 warn' {} \; | sed 's/\// /g' | awk '{ print $2 }'

210115_M06578_0042_000000000-JF7MT_demultiplex 210304_M06578_0044_000000000-JF7WC_demultiplex 210311_M06578_0045_000000000-JF7N3_demultiplex 210317_M06578_0046_000000000-DB8C6_demultiplex 210319_M06578_0047_000000000-JF8D2_demultiplex 210325_M06578_0048_000000000-JF8G4_demultiplex 210415_M06578_0049_000000000-JL3JW_demultiplex 210422_M06578_0050_000000000-JKHD4_demultiplex 210512_M06578_0051_000000000-JL3FM_demultiplex 210521_M06578_0052_000000000-JKW2V_demultiplex 210603_M06578_0053_000000000-JKHBM_demultiplex 210610_M06578_0054_000000000-DC5G2_demultiplex 210611_M06578_0055_000000000-JN9DH_demultiplex 210618_M06578_0056_000000000-JN84N_demultiplex 210623_M06578_0057_000000000-DC4KC_demultiplex 210625_M06578_0058_000000000-JMJN4_demultiplex 210806_M06578_0059_000000000-JN8RR_demultiplex 210813_M06578_0060_000000000-JN8MH_demultiplex 210818_M06578_0061_000000000-DC5DP_demultiplex 210827_M06578_0062_000000000-JR8RK_demultiplex 201218_M06578_0041_000000000-JF7TM_demultiplex 210212_M06578_0043_000000000-DB8DF_demultiplex 210830_M06578_0063_000000000-JRC67_demultiplex 210902_M06578_0064_000000000-DC5Y9_demultiplex 211001_M06578_0067_000000000-JV8DG_demultiplex 211015_M06578_0069_000000000-JVY98_demultiplex 211022_NB552450_0003_AH725HAFX3_demultiplex 220221_M06578_0090_000000000-DFMWT_demultiplex 220422_M06578_0098_000000000-K887T_demultiplex 221103_NB552450_0010_AHJGKKBGXL_demultiplex 220314_M06578_0091_000000000-DFM6K_demultiplex.orig

problem is that the issue came up only once in the old host.

I will look for the same in my workstation copy of the data. If i don't find anything i'll just re-demultiplex everything over a weekend.