recently we have been working on a PBPK model and have some trouble solving an issue with the calculation of clearance for a drug metabolized by CYP3A4. To better comprehend the results we were asking ourselves if - and if yes, then how - it is possible to calculate the clearance upon the following given information:
GFR = 35 mL/min
GFR fraction = 1
specific hepatic clearance = 0.8 1/min
CYP3A4 ontogeny factor = 0.8
CYP3A4 Km = 100 µmol/L
CYP3A4 kcat = 20 1/min
Some, but only few physiological data is known.
Could somebody help us calculate the clearance for this scenario or would you need any other factors such as e.g. fraction unbound?
Dear OPS-community,
recently we have been working on a PBPK model and have some trouble solving an issue with the calculation of clearance for a drug metabolized by CYP3A4. To better comprehend the results we were asking ourselves if - and if yes, then how - it is possible to calculate the clearance upon the following given information:
GFR = 35 mL/min GFR fraction = 1 specific hepatic clearance = 0.8 1/min CYP3A4 ontogeny factor = 0.8 CYP3A4 Km = 100 µmol/L CYP3A4 kcat = 20 1/min
Some, but only few physiological data is known. Could somebody help us calculate the clearance for this scenario or would you need any other factors such as e.g. fraction unbound?
Best regards, Sebastian