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OpenGene / fastp

An ultra-fast all-in-one FASTQ preprocessor (QC/adapters/trimming/filtering/splitting/merging...)
MIT License
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run the same fastp command but get different result of clean fastq files #350

Open tangmaomao16 opened 3 years ago

tangmaomao16 commented 3 years ago

I use Ubuntu 16.04 on a server, use fastp v0.20.1 to do QC, but I find that I get different clean read data files. For example, today 10:00 am I run fastp for raw sequencing data, I get clean fastq files; today 14:00 pm I run the same program again, and I get the clean fastq files of which the contents are different from 10:00 am. Please tell me why and how can I get the same clean fastq files even if I run many times program. The bash command is below: /home/tang/anaconda3/envs/transcript_env/bin/fastp --in1 CF1.R1.raw.fq --in2 CF1.R2.raw.fq --out1 CF1.R1.clean.fq --out2 CF1.R2.clean.fq --thread 4 --trim_front1 2 --trim_front2 2 --cut_front --cut_tail --cut_window_size 3 --cut_mean_quality 30 --json CF1.json --html CF1.html --overrepresentation_analysis

jammywwh commented 1 year ago

hey, did you solve it?

sfchen commented 1 year ago

New version solved this problem