Reads with identical UMIs and mapping coordinates output by Gencore

OpenGene / gencore

Generate duplex/single consensus reads to reduce sequencing noises and remove duplications

MIT License

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Reads with identical UMIs and mapping coordinates output by Gencore #45

Open luederm opened 2 years ago

luederm commented 2 years ago

Hello,

I am running the latest gencore with --no_duplex and -s = 3. However, I am still getting many reads output which have the same UMI and identical mapping coordinates. Please see the IGV screenshot below. Reads are shown as pairs and are grouped by UMI, which can be seen on the left.