I am new to Seurat and scRNA data analysis, so please be kind and don't refer me to other sources I could read for help. I have tried a lot of things and I just want someone to explain what I did wrong before I submit my results to my mentor. I am trying to learn as much as possible on my own first and then I will discuss with her.
I basically had to find differentially expressed genes between Braak 0 and Braak 6 stage patients.
I used the following chunk of code:
I got 465 differentially expressed genes in Braak 6 relative to Braak 0. However, my mentor says she got many more genes on her list. Can someone help me out? What am I doing wrong? I am a little confused.
Hi!
Dataset source: https://cellxgene.cziscience.com/collections/180bff9c-c8a5-4539-b13b-ddbc00d643e6 (I downloaded the second one for practice).
I am new to Seurat and scRNA data analysis, so please be kind and don't refer me to other sources I could read for help. I have tried a lot of things and I just want someone to explain what I did wrong before I submit my results to my mentor. I am trying to learn as much as possible on my own first and then I will discuss with her.
I basically had to find differentially expressed genes between Braak 0 and Braak 6 stage patients. I used the following chunk of code:
I got 465 differentially expressed genes in Braak 6 relative to Braak 0. However, my mentor says she got many more genes on her list. Can someone help me out? What am I doing wrong? I am a little confused.
Thanks!